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首页> 外文期刊>Cell and Tissue Research >Distribution and co-localization of diversified natriuretic peptides in the eel heart
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Distribution and co-localization of diversified natriuretic peptides in the eel heart

机译：鳗鱼心脏多样性利尿肽的分布与共定

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Atrial and B-type natriuretic peptides (ANP and BNP) are cardiac hormones important for cardiovascular and body fluid regulation. In some teleost species, an additional member of the natriuretic peptide family, ventricular NP (VNP), has been identified. In this study, we examine tissue distribution of these three NPs in the eel heart. Quantitative real-time PCR showed that anp is almost exclusively expressed in atria, bnp equally in atria and ventricles and vnp three-fold more in ventricles than in atria. The amount of bnp transcript overall in the heart was 1/10 those of anp and vnp. There was no difference in transcript levels between freshwater and seawater-acclimated fishes. Immunohistochemistry using specific antisera and in situ hybridization using gene-specific probes showed that NP signals were detected in most atrial and ventricular myocytes with some regional differences in density. Because of high sequence similarity of the three NPs, each of the three NP antisera individually was pre-incubated with 10(-8) M of the other two non-targeted cardiac NPs to increase the specificity. A few atrial myocytes contained all three NPs in the same cell. Immuno-electron microscopy identified many dense-core vesicles containing ANP in atria and VNP in ventricles and some vesicles contained both ANP and VNP as demonstrated using pre-absorbed antisera. Based on these data and those of previous studies, we suggest that in eels ANP is secreted from atria in a regulatory pathway and VNP from ventricles in a constitutive pathway. In addition, VNP, not BNP, is the principal ventricular hormone in eels.
机译：心房和B型Natriuretic肽（ANP和BNP）是心血管和体液调节的心脏激素。在一些紧邻的物种中，已经确定了Natriesuretic Peptide系列，室性NP（VNP）的另外的成员。在这项研究中，我们在鳗鱼心脏中检查这三个NP的组织分布。定量实时PCR表明，ANP几乎完全在ATRIA，BNP在Atria和脑室和脑室等VNP中患有比在ATRIA中的3倍。心脏中总体的BNP转录物的量为ANP和VNP的1/10。淡水和海水适应鱼类之间的成绩单水平没有差异。使用特异性抗血清和使用基因特异性探针的免疫组织化学表明，在大多数心房和心室肌细胞中检测到NP信号，其密度的一些区域差异。由于三个NPS的高序列相似性，三个NP抗血清中的每一个与其他两种非靶向心脏NP的10（-8）M单独孵育，以增加特异性。少数心房肌细胞包含在同一细胞中的所有三个NP。免疫电子显微镜鉴定出许多含有ANP的含有ANP的致密核囊泡，VNP在脑室中，一些囊泡含有ANP和VNP，如使用预吸收的抗血清所证明。基于这些数据和先前研究的数据，我们建议在鳗鱼ANP中，从内核途径和来自组成型途径的心室的VNP分泌。此外，VNP不是BNP，是鳗中的主要心室激素。

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