首页> 外文期刊>Cellular microbiology >EhRho1 regulates plasma membrane blebbing through PI3 kinase in Entamoeba histolytica Entamoeba histolyticaEntamoeba histolytica

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EhRho1 regulates plasma membrane blebbing through PI3 kinase in Entamoeba histolytica Entamoeba histolyticaEntamoeba histolytica

机译：EHRHO1通过PI3激酶在 entamoeba组织olytica entamoeba histolytica entamoeba组织olytica中通过pi3激酶进行血浆膜膨胀

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Summary > The protozoan parasite <fc> <fi>Entamoeba histolytica</fi> </fc> causes amoebiasis, a major public health problem in developing countries. Motility of <fc> <fi>E.?histolytica</fi> </fc> is important for its pathogenesis. Blebbing is an essential process contributing to cellular motility in many systems. In mammalian cells, formation of plasma membrane blebs is regulated by Rho‐GTPases through its effectors, such as Rho kinase, mDia1, and acto‐myosin proteins. In this study, we have illuminated the role of EhRho1 in bleb formation and motility of <fc> <fi>E.?histolytica</fi> </fc> . EhRho1 was found at the site of bleb formation in plasma membrane of trophozoites. Overexpression of mutant EhRho1 defective for Guanosine triphosphate (GTP)‐binding or down‐regulating EhRho1 by antisense RNA resulted in reduced blebbing and motility. Moreover, serum‐starvation reduced blebbing that was restored on serum‐replenishment. Lysophosphatidic acid treatment induced bleb formation, whereas wortmannin inhibited the process. In all these cases, concentration of GTP‐EhRho1 (active) and Phosphatidylinositol 4,5‐bisphosphate (PIP2) inversely correlated with the level of plasma membrane blebbing. Our study suggests the role of EhRho1 in blebbing and bleb‐based motility through PI3 kinase pathway in <fc> <fi>E.?histolytica</fi> </fc> . </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”> <标题类型=“main”>摘要</ title> > propozoan parasite <fc> <fi> entamoeba histolytica </ fi> </ fc>导致amoebiasis，一个主要的公共卫生发展中国家的问题。 <Fc> <FI> E.?Histolytica </ Fi> </ Fc>的动力对于其发病机制是重要的。 Blebbing是许多系统中的蜂窝运动的重要过程。在哺乳动物细胞中，通过其效应器（例如Rho激酶，MDIA1和Acto-myosin蛋白）通过rho-gtpases来调节血浆膜脑膜的形成。在这项研究中，我们已经照亮了EHRHO1在<FC> <FI> E.?HISTOLYTICA </ FI> </ FC>的运动中的作用。 Ehrho1被发现在滋养的血浆膜中的BLEB形成部位。突变体EHRHO1的过度表达对鸟嘌呤三磷酸（GTP）的缺陷 - 通过反义RNA缠绕或向下调节EHRHO1导致降低的肺炎和运动。此外，血清饥饿降低了血清补给恢复的肺泡。溶血磷脂酸治疗诱导的BLEB形成，而Wortmannin抑制该过程。在所有这些情况下，GTP-EHRHO1（活性）和磷脂酰肌醇4,5-双磷酸肌醇的浓度与血浆膜膨胀水平逆转。我们的研究表明EHRHO1通过PI3激酶途径在<FC> <FI> E.?HISTOLYTICA </ FI> </ FC>中的作用，通过PI3激酶途径作用。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15891/'>《Cellular microbiology》</a> <b style="margin: 0 2px;">|</b><span>2017年第10期</span><b style="margin: 0 2px;">|</b><span>共18页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bharadwaj Ravi&option=202" target="_blank" rel="nofollow">Bharadwaj Ravi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Arya Ranjana&option=202" target="_blank" rel="nofollow">Arya Ranjana;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shahid mansuri M.&option=202" target="_blank" rel="nofollow">Shahid mansuri M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bhattacharya Sudha&option=202" target="_blank" rel="nofollow">Bhattacharya Sudha;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bhattacharya Alok&option=202" target="_blank" rel="nofollow">Bhattacharya Alok;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of BiotechnologyJawaharlal Nehru UniversityNew Delhi India;</p> <p>School of BiotechnologyJawaharlal Nehru UniversityNew Delhi India;</p> <p>Yale School of MedicineYale UniversityNew Haven USA;</p> <p>School of environmental SciencesJawaharlal Nehru UniversityNew Delhi India;</p> <p>School of Life SciencesJawaharlal Nehru UniversityNew Delhi India;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/173.html" title="普通生物学">普通生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bleb&option=203" rel="nofollow">bleb;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=EhRho1&option=203" rel="nofollow">EhRho1;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Entamoeba histolytica&option=203" rel="nofollow">Entamoeba histolytica;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=PI3 kinases&option=203" rel="nofollow">PI3 kinases;</a> </p> <div class="translation"> 机译：bleb;ehrho1;entamoeba histolytica;pi3激酶; 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