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首页> 外文期刊>Cell cycle >The Lnc LINC00461/miR-30a-5p facilitates progression and malignancy in non-small cell lung cancer via regulating ZEB2
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The Lnc LINC00461/miR-30a-5p facilitates progression and malignancy in non-small cell lung cancer via regulating ZEB2

机译：LNC LINC00461 / MIR-30A-5P通过调节ZEB2促进非小细胞肺癌中的进展和恶性肿瘤

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Studies have found that Lnc LINC00461 is an important regulator of cancer. However, the function of Lnc LINC00461 in NSCLC is not known. Therefore, this experimental design was based on Lnc LINC00461 to explore the pathogenesis of Non-small cell lung cancer (NSCLC). RT-qPCR was used to detect the expression of lnc LINC00461 and miR-30a-5p in NSCLC. The CCK-8 method and Transwell assay were used to detect the effects of lnc LINC00461 and miR-30a-5p on proliferation, migration in NSCLC. Target gene prediction and screening, luciferase reporter assays were used to validate downstream target genes of lnc LINC00461 and miR-30a-5p. The protein expression of ZEB2 was detected by Western blot. The tumor changes in mice were detected by in vivo experiments. Lnc LINC00461 was significantly elevated in NSCLC. Lnc LINC00461 knockdown significantly inhibited proliferation and migration in NSCLC. miR-30a-5p was a direct target of lnc LINC00461 and miR-30a-5p was significantly reduced in NSCLC. shLINC00461 and miR-30a-5p inhibitor partially eliminated the effect of shLINC00461 on cell proliferation. And lnc LINC00461 was negatively correlated with miR-30a-5p expression. ZEB2 was a direct target of miR-30a-5p, and miR-30a-5p mimic and sh lnc LINC00461 significantly reduced ZEB2 expression levels. Finally, In vivo, lnc LINC00461 promoted tumor growth by modulating the miR-30a-5p / ZEB2 axis. In conclusion, LncLINC00461 promoted the progression of NSCLC by the miR-30a-5p / ZEB2 axis, and lnc LINC00461 may be a potential therapeutic target for NSCLC.
机译：研究发现，LNC LINC00461是癌症的重要调节因素。然而，NSCLC中的LNC LINC00461的功能尚不清楚。因此，该实验设计基于LNC LINC00461，探讨非小细胞肺癌（NSCLC）的发病机制。 RT-QPCR用于检测NSCLC中LNC LINC00461和MIR-30A-5P的表达。 CCK-8方法和Transwell测定用于检测LNC LINC00461和MIR-30A-5P对NSCLC迁移的影响。靶基因预测和筛选，荧光素酶报告结果用于验证LNC LINC00461和MIR-30A-5P的下游靶基因。通过蛋白质印迹检测ZeB2的蛋白表达。通过体内实验检测小鼠的肿瘤变化。 LNC LINC00461在NSCLC中显着升高。 LNC LINC00461敲低明显抑制NSCLC的增殖和迁移。 miR-30a-5p是LNC LINC00461的直接靶标，MIR-30a-5p在NSCLC中显着降低。 Shlinc00461和MiR-30A-5P抑制剂部分消除了Shlinc00461对细胞增殖的影响。和LNC LINC00461与miR-30a-5p表达呈负相关。 Zeb2是miR-30a-5p的直接靶标，MiR-30a-5p模拟和Sh lnc linc00461显着降低了Zeb2表达水平。最后，在体内，LNC LINC00461通过调节MIR-30A-5P / ZEB2轴来促进肿瘤生长。总之，LNClinC00461通过MIR-30A-5P / ZEB2轴促进NSCLC的进展，并且LNC LINC00461可以是NSCLC的潜在治疗靶标。

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