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首页> 外文期刊>Cell cycle >MicroRNA-139-5p modulates the growth and metastasis of malignant melanoma cells via the PI3K/AKT signaling pathway by binding to IGF1R
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MicroRNA-139-5p modulates the growth and metastasis of malignant melanoma cells via the PI3K/AKT signaling pathway by binding to IGF1R

机译：MicroRNA-139-5P通过与IGF1R结合，通过PI3K / AKT信号通路调节恶性黑素瘤细胞的生长和转移

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摘要

The relation between microRNAs (miRNAs) and malignant melanoma has been demonstrated in previous studies, while there was little research about miR-139-5p and malignant melanoma. The aim of this study is to investigate the ability of miR-139-5p in malignant melanoma cells via the modulation of the PI3K/AKT signaling pathway by targeting IGF1R. MiR-139-5p expression in malignant melanoma tissues and 5 malignant melanoma cell lines was detected. The melanoma cells were transfected with miR-139-5p mimic negative control (NC) sequence, miR-139-5p mimic, IGF1R overexpressed recombinant plasmid NC or IGF1R overexpressed sequence. The expression of Akt signaling pathway-related protein was evaluated. The biological functions in malignant melanoma cells were evaluated by a string of experiments. MiR-139-5p expressed a poor level in tissues and cell lines of malignant melanoma. Overexpressed miR-139-5p suppressed the cell proliferation, migration, and invasion, and contributed to the promoted apoptosis of malignant melanoma cells by decreasing IGF1R. MiR-139-5p down-regulated the IGF1R expression, and IGF1R accelerated the activation of the PI3K/AKT signaling pathway. miR-139-5p reversed the promotive impacts of IGF1R on the PI3K/AKT signaling pathway. The study validates that miR-139-5p could suppress malignant melanoma progression through the repression of the PI3K/AKT signaling pathway by downregulating IGF1R. Therefore, miR-139-5p could pave a new way for the treatment of malignant melanoma.
机译：在以前的研究中已经证明了MicroRNA（miRNA）和恶性黑素瘤之间的关系，而关于miR-139-5p和恶性黑素瘤几乎没有研究。本研究的目的是通过靶向IGF1R来研究通过调节PI3K / AKT信号通路的恶性黑素瘤细胞miR-139-5p的能力。检测到MiR-139-5P在恶性黑素瘤组织和5种恶性黑色素瘤细胞中的表达。用miR-139-5P模拟阴性对照（NC）序列，MiR-139-5P模拟，IGF1R过表达重组质粒NC或IGF1R过表达序列转染黑色素瘤细胞。评估AKT信号传导途径相关蛋白的表达。通过一系列实验评估恶性黑素瘤细胞的生物学功能。 miR-139-5p在恶性黑素瘤的组织和细胞系中表达了差的水平。过表达MIR-139-5P抑制了细胞增殖，迁移和侵袭，并通过降低IGF1R促进恶性黑素瘤细胞的促进凋亡。 MiR-139-5P下调IGF1R表达，IGF1R加速了PI3K / AKT信号通路的激活。 MiR-139-5P反转IGF1R对PI3K / AKT信号通路的促进影响。该研究验证MIR-139-5P可以通过下调IGF1R抑制PI3K / AKT信号通路的抑制恶性黑色素瘤进展。因此，MIR-139-5P可以为治疗恶性黑素瘤铺平新途径。

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  • 来源
    《Cell cycle》 |2019年第24期|共12页
  • 作者

    Yang Chaoying; Xia Zhaoxia; Zhu Lifei; Li Yanchang; Zheng Zhixin; Liang Jianying; Wu Liangcai;

  • 作者单位

    Sun Yat SenUniv Affiliated Hosp 6 Dept Dermatol Guangzhou Peoples R China;

    Sun Yat SenUniv Affiliated Hosp 6 Dept Ophthalmol Guangzhou Peoples R China;

    Sun Yat SenUniv Affiliated Hosp 6 Dept Dermatol Guangzhou Peoples R China;

    Sun Yat SenUniv Affiliated Hosp 6 Dept Dermatol Guangzhou Peoples R China;

    Sun Yat SenUniv Affiliated Hosp 6 Dept Dermatol Guangzhou Peoples R China;

    Shanghai Jiao Tong Univ Xinhua Hosp Sch Med Dept Dermatol Shanghai Peoples R China;

    Sun Yat SenUniv Affiliated Hosp 6 Dept Dermatol Guangzhou Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    MicroRNA-139-5p; IGF1R; PI3K/AKT signaling pathway;

    机译：MicroRNA-139-5P;IGF1R;PI3K / AKT信号通路;

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