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Construction and analysis of circular RNA molecular regulatory networks in liver cancer

机译：肝癌圆形RNA分子调控网络的构建与分析

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摘要

Liver cancer is the sixth most prevalent cancer, and the third most frequent cause of cancer-related deaths. Circular RNAs (circRNAs), a kind of special endogenous ncRNAs, have been coming back to the forefront of cancer genomics research. In this study, we used a systems biology approach to construct and analyze the circRNA molecular regulatory networks in the context of liver cancer. We detected a total of 127 differentially expressed circRNAs and 3,235 differentially expressed mRNAs. We selected the top-5 upregulated circRNAs to construct a circRNA-miRNA-mRNA network. We enriched the pathways and gene ontology items and determined their participation in cancer-related pathways such as p53 signaling pathway and pathways involved in angiogenesis and cell cycle. Quantitative real-time PCR was performed to verify the top-five circRNAs. ROC analysis showed circZFR, circFUT8, circIPO11 could significantly distinguish the cancer samples, with an AUC of 0.7069, 0.7575, and 0.7103, respectively. Our results suggest the circRNA-miRNA-mRNA network may help us further understand the molecular mechanisms of tumor progression in liver cancer, and reveal novel biomarkers and therapeutic targets.
机译：肝癌是第六次最普遍的癌症，以及癌症相关死亡的第三种最常见的原因。圆形rnas（circrnas），一种特殊的内源性Ncrnas，已经回到了癌症基因组学研究的最前沿。在这项研究中，我们使用了系统生物学方法来构建和分析肝癌背景下的CircrNA分子调节网络。我们检测到总共127个差异表达的Circrnas和3,235个差异表达的MRNA。我们选择了前5个上调Circrnas来构建Circrna-miRNA-mRNA网络。我们富集了途径和基因本体项目，并确定了他们参与癌症相关的途径，例如P53信号传导途径和参与血管生成和细胞周期的途径。进行定量实时PCR以验证前五个Circrnas。 ROC分析显示Circzfr，CircFut8，Circipo11可以显着区分癌症样本，分别为0.7069,0.7575和0.7103的AUC。我们的结果表明Circrna-miRNA-mRNA网络可以帮助我们进一步了解肝癌中肿瘤进展的分子机制，并揭示新型生物标志物和治疗靶标。

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  • 来源
    《Cell cycle》 |2017年第22期|共8页
  • 作者

    Ren Shuangchun; Xin Zhuoyuan; Xu Yinyan; Xu Jianting; Wang Guoqing;

  • 作者单位

    Jilin Univ Coll Basic Med Sci Minist Educ Key Lab Bion Engn Changchun Jilin Peoples R China;

    Jilin Univ Coll Basic Med Sci Minist Educ Key Lab Bion Engn Changchun Jilin Peoples R China;

    Jilin Univ Coll Basic Med Sci Minist Educ Key Lab Bion Engn Changchun Jilin Peoples R China;

    Jilin Univ Hosp 1 Canc Ctr Changchun Jilin Peoples R China;

    Jilin Univ Coll Basic Med Sci Minist Educ Key Lab Bion Engn Changchun Jilin Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    CircRNAs; circRNA-miRNA-mRNA; expression profile; liver cancer;

    机译：Circrnas;circrna-miRNA-mRNA;表达谱;肝癌;

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