• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell cycle >Roles of the checkpoint sensor clamp Rad9-Rad1-Hus1 (911)-complex and the clamp loaders Rad17-RFC and Ctf18-RFC in Schizosaccharomyces pombe telomere maintenance.
【24h】

Roles of the checkpoint sensor clamp Rad9-Rad1-Hus1 (911)-complex and the clamp loaders Rad17-RFC and Ctf18-RFC in Schizosaccharomyces pombe telomere maintenance.

机译：检查点传感器钳位RAD9-RAD1-HUS1（911）的角色 - 复合和夹紧装载机RAD17-RFC和CTF18-RFC在Schizosaccharomyces Pombe Telermere维护中。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

While telomeres must provide mechanisms to prevent DNA repair and DNA damage checkpoint factors from fusing chromosome ends and causing permanent cell cycle arrest, these factors associate with functional telomeres and play critical roles in the maintenance of telomeres. Previous studies have established that Tel1 (ATM) and Rad3 (ATR) kinases play redundant but essential roles for telomere maintenance in fission yeast. In addition, the Rad9-Rad1-Hus1 (911) and Rad17-RFC complexes work downstream of Rad3 (ATR) in fission yeast telomere maintenance. Here, we investigated how 911, Rad17-RFC and another RFC-like complex Ctf18-RFC contribute to telomere maintenance in fission yeast cells lacking Tel1 and carrying a novel hypomorphic allele of rad3 (DBD-rad3), generated by the fusion between the DNA binding domain (DBD) of the fission yeast telomere capping protein Pot1 and Rad3. Our investigations have uncovered a surprising redundancy for Rad9 and Hus1 in allowing Rad1 to contribute to telomere maintenance in DBD-rad3 tel1Delta cells. In addition, we found that Rad17-RFC and Ctf18-RFC carry out redundant telomere maintenance functions in DBD-rad3 tel1Delta cells. Since checkpoint sensor proteins are highly conserved, genetic redundancies uncovered here may be relevant to telomere maintenance and detection of DNA damage in other eukaryotes.
机译：虽然端粒体必须提供防止DNA修复和DNA损伤检查点因子的机制，但导致永久细胞循环骤停，这些因素与功能全粒术语相关，并在维持端粒的维持中发挥关键作用。以前的研究已经建立了Tel1（ATM）和RAD3（ATR）激酶在裂变酵母中对端粒性维持起多余但基本的作用。另外，RAD9-RAD1-HUS1（911）和RAD17-RFC复合物在裂变酵母端粒体维持下的RAD3（ATR）下游工作。在这里，我们研究了911，RAD17-RFC和另一RFC样复合CTF18-RFC在缺乏TEL1的裂变酵母细胞中有助于端粒维持，并携带RAD3（DBD-RAD3）的新的低晶体等位基因，由DNA之间的融合产生裂变酵母端粒封装蛋白POT1和RAD3的结合结构域（DBD）。我们的调查发现RAD9和HUS1的令人惊讶的冗余允许RAD1在DBD-RAD3 TEL1DELTA细胞中有助于端粒维护。此外，我们发现RAD17-RFC和CTF18-RFC在DBD-RAD3 TEL1DELTA细胞中进行冗余的端粒维护功能。由于检查点传感器蛋白质高度保守，因此这里未发现的遗传冗余可能与其他真核生物中的DNA损伤的重组维护和检测相关。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号