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首页> 外文期刊>Cell cycle >Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM.
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Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM.

机译：Xenopus DNA2是一种氦酶/核酸酶，其在复合物中发现，复合蛋白和-1 / CTF4和MCM10和DSB反应蛋白NBS1和ATM。

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We have used the Xenopus laevis egg extract system to study the roles of vertebrate Dna2 in DNA replication and double-strand-break (DSB) repair. We first establish that Xenopus Dna2 is a helicase, as well as a nuclease. We further show that Dna2 is a nuclear protein that is actively recruited to DNA only after replication origin licensing. Dna2 co-localizes in foci with RPA and is found in a complex with replication fork components And-1 and Mcm10. Dna2 interacts with the DSB repair and checkpoint proteins Nbs1 and ATM. We also determine the order of arrival of ATM, MRN, Dna2, TopBP1 and RPA to duplex DNA ends and show that it is the same both in S phase and M phase extracts. Interestingly, Dna2 can bind to DNA ends independently of MRN, but efficient nucleolytic resection, as measured by RPA recruitment, requires both MRN and Dna2. The nuclease activity of Mre11 is required, since its inhibition delays both full Dna2 recruitment and resection. Dna2 depletion inhibits but does not block resection, and Chk1 and Chk2 induction occurs in the absence of Dna2.
机译：我们使用了Xenopus Laevis蛋提取系统来研究脊椎动物DNA2在DNA复制中的作用和双链 - 断裂（DSB）修复。我们首先建立Xenopus DNA2是螺旋酶，以及核酸酶。我们进一步表明，DNA2是仅在复制源许可后积极募集到DNA的核蛋白质。 DNA2与RPA共定位焦灶片，并在复合物中发现，复制叉组件和-1和MCM10。 DNA2与DSB修复和检查点蛋白NBS1和ATM相互作用。我们还确定ATM，MRN，DNA2，TOPBP1和RPA到双相DNA的到达顺序结束，并表明它在S期和M相提取物中是相同的。有趣的是，DNA2可以与MRN独立地与DNA结合，但是通过RPA募集测量的高效核渗透切除，需要MRN和DNA2。需要MRE11的核酸酶活性，因为其抑制延迟全DNA2招生和切除。 DNA2耗竭抑制但不堵塞切除，并且在没有DNA2的情况下发生CHK1和CHK2诱导。

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