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首页> 外文期刊>Cell cycle >Involvement of LIMK1/2 in actin assembly during mouse embryo development
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Involvement of LIMK1/2 in actin assembly during mouse embryo development

机译：Limk1 / 2在小鼠胚胎发育过程中的肌动蛋白组装中的参与

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摘要

LIMKs (LIMK1 and LIMK2) are serine/threonine protein kinases that involve in various cellular activities such as cell migration, morphogenesis and cytokinesis. However, its roles during mammalian early embryo development are still unclear. In the present study, we disrupted LIMK1/2 activity to explore the functions of LIMK1/2 during mouse early embryo development. We found that p-LIMK1/2 mainly located at the cortex of each blastomeres from 2-cell to 8-cell stage, and p-LIMK1/2 also expressed at morula and blastocyst stage in mouse embryos. Inhibition of LIMK1/2 activity by LIMKi 3 (BMS-5) at the zygote stage caused the failure of embryo early cleavage, and the disruption of LIMK1/2 activity at 8-cell stage caused the defects of embryo compaction and blastocyst formation. Fluorescence staining and intensity analysis results demonstrated that the inhibition of LIMK1/2 activity caused aberrant cortex actin expression and the decrease of phosphorylated cofilin in mouse embryos. Taken together, we identified LIMK1/2 as an important regulator for cofilin phosphorylation and actin assembly during mouse early embryo development.
机译：跛行（利润率和利润2）是丝氨酸/苏氨酸蛋白激酶，其涉及各种细胞活性，例如细胞迁移，形态发生和细胞因子。然而，它在哺乳动物早期胚胎发育中的作用仍然不明确。在本研究中，我们扰乱了Limk1 / 2的活性，以探讨小鼠早期胚胎发育期间利用Limk1 / 2的功能。我们发现P-LiMK1 / 2主要位于来自2细胞至8个细胞阶段的每个卵囊的皮质，并且P-Limk1 / 2也在小鼠胚胎中在森拉和胚泡阶段表达。利用阶段抑制Limki 3（BMS-5）的Limk1 / 2活性导致胚胎早期切割的失败，8细胞阶段的Limk1 / 2活性的破坏引起了胚胎压实和胚泡形成的缺陷。荧光染色和强度分析结果表明，LiMK1 / 2活性的抑制引起了异常皮质肌动蛋白的表达和小鼠胚胎中磷酸化钴蛋白的降低。我们一起携带，我们将LiMK1 / 2鉴定为辛硅磷酸化和小鼠早期胚胎发育过程中肌肉组装的重要调节剂。

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  • 来源
    《Cell cycle》 |2018年第11期|共9页
  • 作者

    Duan Xing; Zhang Hao-Lin; Wu Lan-Lan; Liu Meng-Yao; Pan Meng-Hao; Ou Xiang-Hong; Sun Shao-Chen;

  • 作者单位

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing Jiangsu Peoples R China;

    Guangdong Second Prov Gen Hosp Reprod Med Ctr Fertil Preservat Lab Guangzhou Guangdong Peoples;

    Nanjing Agr Univ Coll Anim Sci &

    Technol Nanjing Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    LIMK1/2; actin; embryo development; blastocyst;

    机译：Limk1 / 2;actin;胚胎发育;胚泡;

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