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首页> 外文期刊>Cell cycle >Microarray expression profile and analysis of circular RNA regulatory network in malignant pleural effusion
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Microarray expression profile and analysis of circular RNA regulatory network in malignant pleural effusion

机译：微阵列表达谱与恶性胸腔积液中圆形RNA调节网络的分析

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Malignant pleural effusion (MPE) is a common complication of lung cancer. Accumulating evidence has suggested that circular RNAs (circRNAs) play important roles in oncogenesis and progression of cancer. However, the expression pattern of circRNAs in MPE remains largely unknown and awaits investigation. The study was designed to elucidate the potential roles of differentially expressed circRNAs in MPE. Herein, we detected a total of 1350 differentially expressed circRNAs and 1727 differentially expressed mRNAs in lung adenocarcinoma-associated malignant pleural effusion (LA-MPE) compared with tuberculous pleural effusion (TPE) by Clariom D Human Microarray. Among the top 5 up-regulated circRNAs (hsa_circ_0067705, hsa_circ_0025542, hsa_circ_0072793, hsa_circ_0084927, and hsa_circ_0085386), four were verified significantly up-regulated in LA-MPE by qRT-PCR and hsa_circ_0085386 had an increasing trend. CircRNA-miRNA-mRNA network for the top 5 up-regulated circRNAs was constructed and pathway analysis indicated that the enriched mRNA targets involved in PI3K-Akt signaling pathway, Axon guidance, Regulation of actin cytoskeleton and Rap1 signaling pathway were potentially regulated by these aberrantly expressed circRNAs. We generated specific circRNA profiles in LA-MPE for the first time. And analysis of circRNA regulatory network could provide evidence that circRNAs are important in MPE development because they participate in cancer-related pathways by sequestering miRNAs. Our findings suggested that aberrantly expressed circRNAs may be involved in the development of LA-MPE.
机译：恶性胸腔积液（MPE）是肺癌的常见并发症。积累证据表明，圆形RNA（Circrnas）在癌症和癌症进展中起重要作用。然而，MPE中Circrnas的表达模式仍然很大程度上是未知的并且等待调查。该研究旨在阐明差异表达Circrnas在MPE中的潜在作用。在此，与Clariom D人微阵列相比，我们检测到总共1350个差异表达的CircrNA和1727型肺腺癌相关恶性胸腔积液（La-MPE）中的MRNA。在前5个上调的Circrnas（HSA_CIRC_0067705，HSA_CIRC_0025542，HSA_CIRC_0072793，HSA_CIRC_0084927和HSA_CIRC_0085386）中，通过QRT-PCR在LA-MPE中显着上调，HSA_CIRC_0085386具有越来越多的趋势。构建了前五个上调CircrNA的Circrna-miRNA-mRNA网络，并且途径分析表明，参与PI3K-AKT信号通路的富集MRNA靶，轴突引导，肌动蛋白细胞骨架和RAP1信号传导途径的调节潜在地受到这些异常调节表达Circrnas。我们首次在La-MPE中生成特定的CircrNA配置文件。 Circrna监管网络的分析可以提供证据，即Circrnas在MPE开发中是重要的，因为它们通过螯合MiRNA参与癌症相关的途径。我们的研究结果表明，异常表达的Circrnas可能参与La-MPE的发展。

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