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首页> 外文期刊>Cell Proliferation >Single cell-derived clonally expanded mesenchymal progenitor cells from somatic cell nuclear transfer-derived pluripotent stem cells ameliorate the endometrial function in the uterus of a murine model with Asherman's syndrome
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Single cell-derived clonally expanded mesenchymal progenitor cells from somatic cell nuclear transfer-derived pluripotent stem cells ameliorate the endometrial function in the uterus of a murine model with Asherman's syndrome

机译：来自体细胞核转移衍生多能干细胞的单细胞衍生的克隆性祖细胞祖细胞改善了用芪综合征的鼠模型子宫内膜功能

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Objectives Because primary mesenchymal progenitor cells (adult-MPCs) have various functions that depend on the tissue origin and donor, de novo MPCs from human pluripotent stem cells (hPSCs) would be required in regenerative medicine. However, the characteristics and function of MPCs derived from reprogrammed hPSCs have not been well studied. Thus, we show that functional MPCs can be successfully established from a single cell-derived clonal expansion following MPC derivation from somatic cell nuclear transfer-derived (SCNT)-hPSCs, and these cells can serve as therapeutic contributors in an animal model of Asherman's syndrome (AS). Materials and methods We developed single cell-derived clonal expansion following MPC derivation from SCNT-hPSCs to offer a pure population and a higher biological activity. Additionally, we investigated the therapeutic effects of SCNT-hPSC-MPCs in model mice of Asherman's syndrome (AS), which is characterized by synechiae or fibrosis with endometrial injury. Results Their humoral effects in proliferating host cells encouraged angiogenesis and decreased pro-inflammatory factors via a host-dependent mechanism, resulting in reduction in AS. We also addressed that cellular activities such as the cell proliferation and population doubling of SCNT-hPSC-MPCs resemble those of human embryonic stem cell-derived MPCs (hESC-MPCs) and are much higher than those of adult-MPCs. Conclusions Somatic cell nuclear transfer-derived-hPSCs-MPCs could be an advanced therapeutic strategy for specific diseases in the field of regenerative medicine.
机译：目标是因为初级间充质祖细胞（成人MPC）具有依赖于组织来源和供体的各种功能，因此在再生医学中需要来自人多能干细胞（HPSC）的NovoMPC。然而，来自重编程的HPSCS的MPC的特性和功能尚未得到很好的研究。因此，我们表明，可以从体细胞核转移衍生（SCNT）-HPSCS的MPC衍生后，从单个细胞衍生的克隆膨胀中成功地建立功能MPC，并且这些细胞可以作为Asherman综合征的动物模型中的治疗贡献者（作为）。材料和方法我们开发了来自SCNT-HPSCS的MPC衍生后的单细胞衍生克隆膨胀，以提供纯粹的群体和更高的生物活性。此外，我们研究了SCNT-HPSC-MPC在伊赫曼综合征（AS）模型中的治疗效果，其特征在于具有子宫内膜损伤的联系或纤维化。导致它们在增殖宿主细胞中的体液效应促进血管生成和通过宿主依赖机制降低的促炎因子，导致减少为。我们还解决了细胞活性，例如SCNT-HPSC-MPCs的细胞增殖和群体倍增，类似于人胚胎干细胞衍生的MPCs（HESC-MPC），并且远高于成人MPC。结论躯体细胞核转移衍生 - HPSCS-MPC可以是再生医学领域的特定疾病的先进治疗策略。

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