Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution

Fletcher Russell B.; Das Diya; Gadye Levi; Street Kelly N.; Baudhuin Ariane; Wagner Allon; Cole Michael B.; Flores Quetzal; Choi Yoon Gi; Yosef Nir; Purdom Elizabeth; Dudoit Sandrine; Risso Davide; Ngai John

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Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution

机译：单细胞分辨率解构嗅觉干细胞轨迹

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A detailed understanding of the paths that stem cells traverse to generate mature progeny is vital for elucidating the mechanisms governing cell fate decisions and tissue homeostasis. Adult stem cells maintain and regenerate multiple mature cell lineages in the olfactory epithelium. Here we integrate single-cell RNA sequencing and robust statistical analyses with in vivo lineage tracing to define a detailed map of the postnatal olfactory epithelium, revealing cell fate potentials and branchpoints in olfactory stem cell lineage trajectories. Olfactory stem cells produce support cells via direct fate conversion in the absence of cell division, and their multipotency at the population level reflects collective unipotent cell fate decisions by single stem cells. We further demonstrate that Wnt signaling regulates stem cell fate by promoting neuronal fate choices. This integrated approach reveals the mechanisms guiding olfactory lineage trajectories and provides a model for deconstructing similar hierarchies in other stem cell niches.

机译：详细了解干细胞横移以产生成熟后代的途径对于阐明控制细胞命运决策和组织稳态的机制至关重要。成体干细胞在嗅觉上皮中维持和再生多种成熟细胞谱系。在这里，我们将单细胞RNA测序和强大的统计分析与体内谱系追踪集成，以定义产后嗅觉上皮的详细地图，揭示嗅干细胞谱系轨迹中的细胞命运电位和分支。嗅觉干细胞通过在没有细胞分裂的情况下通过直接命运转化产生载体细胞，并且它们在人口水平的多种性反映了单干细胞的集体单能细胞命运决策。我们进一步证明WNT信号传导通过促进神经元命运选择来调节干细胞命运。这种综合方法揭示了引导嗅觉轨迹轨迹的机制，并提供用于在其他干细胞利基中解构类似层次结构的模型。

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《Cell stem cell》 |2017年第6期|共22页
作者
Fletcher Russell B.; Das Diya; Gadye Levi; Street Kelly N.; Baudhuin Ariane; Wagner Allon; Cole Michael B.; Flores Quetzal; Choi Yoon Gi; Yosef Nir; Purdom Elizabeth; Dudoit Sandrine; Risso Davide; Ngai John;
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作者单位

Univ Calif Berkeley Dept Mol &

Cell Biol Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Mol &

Cell Biol Berkeley CA 94720 USA;

Univ Calif Berkeley Helen Wills Neurosci Inst Berkeley CA 94720 USA;

Univ Calif Berkeley Div Biostat Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Mol &

Cell Biol Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Elect Engn &

Comp Sci Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Phys Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Mol &

Cell Biol Berkeley CA 94720 USA;

Univ Calif Berkeley Funct Genom Lab QB3 Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Elect Engn &

Comp Sci Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Stat Berkeley CA 94720 USA;

Univ Calif Berkeley Div Biostat Berkeley CA 94720 USA;

Univ Calif Berkeley Div Biostat Berkeley CA 94720 USA;

Univ Calif Berkeley Dept Mol &

Cell Biol Berkeley CA 94720 USA;

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正文语种 eng
中图分类细胞生物学;
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专利

1. Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution [J] . Fletcher Russell B., Das Diya, Gadye Levi, Cell stem cell . 2017,第6期

机译：单细胞分辨率解构嗅觉干细胞轨迹
2. Deconstructing stem cell population heterogeneity: Single-cell analysis and modeling approaches [J] . Wu Jincheng, Tzanakakis Emmanuel S. Biotechnology Advances: An International Review Journal . 2013,第7期

机译：解构干细胞种群异质性：单细胞分析和建模方法
3. Mapping lung cancer epithelial-mesenchymal transition states and trajectories with single-cell resolution [J] . Loukia G. Karacosta, Benedict Anchang, Nikolaos Ignatiadis, Nature Communications . 2019,第1期

机译：用单细胞分辨率测绘肺癌上皮 - 间充质转换状态和轨迹
4. 2-D Microfluidic Chemostat System for Long-Term Tracking of E. coli in Growing Population with Single-Cell Resolution [C] . Qiao Ge, rnZhongshi Tang, rnHaiwei Wang Proceedings of the First Shenyang International Colloquium on Microfluidies . 2007

机译：二维微流控化学恒温器系统，用于以单细胞分辨率长期追踪人口增长中的大肠杆菌
5. Analyzing olfactory neurogenesis at single-cell resolution. [D] . Tietjen, Ian William. 2003

机译：以单细胞分辨率分析嗅觉神经发生。
6. Deconstructing Olfactory Stem Cell Trajectories at Single Cell Resolution [O] . Russell B. Fletcher, Diya Das, Levi Gadye, -1

机译：在单细胞分辨率下解构嗅觉干细胞轨迹。
7. Stem cell differentiation trajectories in Hydra resolved at single-cell resolution [O] . Stefan Siebert, Jeffrey A. Farrell, Jack F. Cazet, 2019

机译：Hydra的干细胞分化轨迹在单细胞分辨率下解决

Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution

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