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首页> 外文期刊>Cellular Signalling >TNF alpha-Erk1/2 signaling pathway-regulated SerpinE1 and SerpinB2 are involved in lipopolysaccharide-induced porcine granulosa cell proliferation
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TNF alpha-Erk1/2 signaling pathway-regulated SerpinE1 and SerpinB2 are involved in lipopolysaccharide-induced porcine granulosa cell proliferation

机译：TNFα-ERK1 / 2信号通路调节的蛇纹石1和SerpinB2涉及脂多糖诱导的猪甘蓝细胞增殖

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摘要

Lipopolysaccharide (LPS) is an inhibitory factor that causes hormonal imbalance and subsequently affects ovarian function and fertility in mammals. Previous studies have shown that the exposure of granulosa cells (GC) to LPS leads to steroidogenesis dysfunction. However, the effects of LPS on the viability of GC remain largely unclear. In the present study, we aimed to address this question and unveil the underlying molecular mechanisms using cultured porcine GC. Results showed that GC proliferation and tumor necrosis factor alpha (TNF alpha) secretion were significantly increased after exposure to LPS, and these effects were completely reversed by blocking the TNF alpha sheddase, ADAM17. Moreover, GC proliferation induced by LPS was mimicked by treatment with recombinant TNF alpha. In addition, SerpinE1 and SerpinB2 expression levels increased in GC after treatment with LPS or recombinant TNF alpha, whereas blocking the Erk1/2 pathway completely abolished these effects and also inhibited GC proliferation. Further, consistent with the effects of blocking the Erk1/2 pathway, cell proliferation was completely inhibited by knocking down SerpinE1 or SerpinB2 in the presence of LPS or recombinant TNF alpha. Mitochondrial membrane potential (MMP) polarization in GC was increased by LPS or recombinant TNF alpha treatment, and these changes were completely negated by Erk1/2 inhibition, but not by SerpinE1 or SerpinB2 knockdown. Taken together, these results suggested that the TNF alpha-mediated upregulation of SerpinE1 and SerpinB2, through activation of the Erk1/2 pathway plays a crucial role in LPS-stimulated GC proliferation, and the increase in GC MMP may synergistically influence this process.
机译：脂多糖（LPS）是引起激素不平衡的抑制因素，随后影响哺乳动物的卵巢功能和生育能力。以前的研究表明，将颗粒细胞（GC）暴露于LPS导致甾体过磷功能障碍。然而，LPS对GC活力的影响仍然很大程度上不清楚。在本研究中，我们旨在解决使用培养的猪GC的潜在的分子机制来解决这个问题。结果表明，暴露于LP后，GC增殖和肿瘤坏死因子α（TNFα）分泌显着增加，通过阻断TNFα血液17，完全逆转这些效应。此外，通过用重组TNFα处理LPS诱导的GC增殖。此外，用LPS或重组TNFα处理后GC中的Serpine1和SerpinB2表达水平增加，而阻塞ERK1 / 2途径完全废除这些效果并抑制了GC增殖。此外，与阻断ERK1 / 2途径的影响一致，通过在LPS或重组TNFα存在下敲下蛇碱1或SerpinB2来完全抑制细胞增殖。通过LPS或重组TNFα处理增加了GC中的线粒体膜电位（MMP）偏振，并且通过ERK1 / 2抑制完全否定这些变化，但不能通过血肽1或SerpinB2敲低。这些结果表明，通过激活ERK1 / 2途径的TNFα1和SerpinB2的TNFα-介导的上调在LPS刺激的GC增殖中起着至关重要的作用，并且GC MMP的增加可能协同影响该过程。

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  • 来源
    《Cellular Signalling》 |2020年第1期|共11页
  • 作者

    Qu Xiaolu; Guo Shuangshuang; Yan Leyan; Zhu Huanxi; Li Hui; Shi Zhendan;

  • 作者单位

    Jilin Agr Univ Coll Anim Sci &

    Technol Changchun 130118 Peoples R China;

    Jiangsu Acad Agr Sci Minist Sci &

    Technol State Key Lab Cultivat Base Jiangsu Key Lab Food Qual &

    Safety Nanjing 210014 Peoples R China;

    Jiangsu Acad Agr Sci Minist Sci &

    Technol State Key Lab Cultivat Base Jiangsu Key Lab Food Qual &

    Safety Nanjing 210014 Peoples R China;

    Jiangsu Acad Agr Sci Minist Sci &

    Technol State Key Lab Cultivat Base Jiangsu Key Lab Food Qual &

    Safety Nanjing 210014 Peoples R China;

    Jiangsu Acad Agr Sci Minist Sci &

    Technol State Key Lab Cultivat Base Jiangsu Key Lab Food Qual &

    Safety Nanjing 210014 Peoples R China;

    Jiangsu Acad Agr Sci Minist Sci &

    Technol State Key Lab Cultivat Base Jiangsu Key Lab Food Qual &

    Safety Nanjing 210014 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞形态学;
  • 关键词

    LPS; Granulosa cell; Proliferation; SerpinE1; SerpinB2;

    机译：LPS;甘蓝细胞;增殖;蛇胺1;SerpinB2;

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