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首页> 外文期刊>Cellular Signalling >Mutant ACTB mRNA 3 '-UTR promotes hepatocellular carcinoma development by regulating miR-1 and miR-29a
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Mutant ACTB mRNA 3 '-UTR promotes hepatocellular carcinoma development by regulating miR-1 and miR-29a

机译：突变体ActB mRNA 3'-UTR通过调节miR-1和miR-29a来促进肝细胞癌发育

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In recent years, studies demonstrate that ACTB has been found to be associated with various tumors. Although ACTB is dysregulated in numerous cancer types, limited data are available on the potential function and mechanism of ACTB in hepatocellular carcinoma (HCC). This study evaluated the expression and biological roles of mutant ACTS mRNA 3'-UTR in HCC. Transcriptome sequence and qRT-PCR analysis determined that mutant ACTB nsRNA '-UTR was high expression in tumor tissues. Luciferase reporter assay showed that the ACTS mRNA 3'-UTR mutations made it easier to interact with miR-1 and miR-29a. Moreover, mutant ACTB mRNA '-UTR regulated miR-1 and miR-29a degradation via AGO2. Furthermore, mutant ACTB mRNA 3'-UTR promoted hepatocellular carcinoma cells migration and invasion in vitro and in vivo by up-regulating miR-1 target gene MET and miR-29a target gene MCL1. In a word, our study demonstrates that 3'-UTR of ACTB plays a key role in the development of hepatocellular carcinoma (HCC) and highlights the molecular mechanisms underlying such a complex process.
机译：近年来，研究表明，actb已被发现与各种肿瘤有关。虽然ACTB在许多癌症类型中具有失调，但有限的数据可用于肝细胞癌（HCC）中ACTB的潜在功能和机制。本研究评估了突变作用MRNA 3'-UTR在HCC中的表达和生物学作用。转录组序列和QRT-PCR分析确定突变体ACTB NSRNA'-UTR在肿瘤组织中是高表达。荧光素酶报告器测定表明，作用mRNA 3'-UTR突变使其更容易与miR-1和miR-29a相互作用。此外，突变体ActB mRNA' -UTR调节的miR-1和miR-29a降解通过前缺失。此外，突变体Actb mRNA 3'-UTR在体外促进肝细胞癌细胞迁移和侵袭，并通过上调miR-1靶基因满足和miR-29a靶基因Mcl1。总之，我们的研究表明，Actb的3'UTR在肝细胞癌（HCC）的发展中起着关键作用，并突出了如此复杂的过程所依赖的分子机制。

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