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首页> 外文期刊>Cellular and Molecular Bioengineering >Effect of Sintering Temperature of Bioactive Glass Nanoceramics on the Hemolytic Activity and Oxidative Stress Biomarkers in Erythrocytes
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Effect of Sintering Temperature of Bioactive Glass Nanoceramics on the Hemolytic Activity and Oxidative Stress Biomarkers in Erythrocytes

机译：生物活性玻璃纳米胺烧结温度对红细胞溶血性活性和氧化应激生物标志物的影响

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Introduction The nature of the surface is critical in determining the biological activity of silica powders. A novel correlation between toxicity and surface properties of bioactive glass ceramics (BGCs) synthesized via the sol-gel method was attempted in this study. Methods The behavior of BGCs after their attachment to the surface of red blood cells (RBCs) was evaluated and their toxic effects were determined based on hemolysis, membrane injury via anti-phosphotyrosine immunoblot of Band 3, lipid peroxidation, potential to generate reactive oxygen species, and antioxidant enzyme production. In particular, three BGCs were synthesized and treated at three sintering temperatures (T1 = 835 degrees C, T2 = 1000 degrees C and T3 = 1100 degrees C) to investigate possible relation between surface charge or structure and hemolytic potential. Results Their toxicity based on hemolysis was dose dependent, while BGC-T2 had the best hemocompatibility in compare with the other BGCs.No BGCs in dosages lower than 0.125 mg/mL could damage erythrocytes. On the other hand, all BGCs promoted the production of reactive oxygen species in certain concentrations, with the BGC-T2 producing the lowest ROS and increasing the glutathione levels in RBCs protecting their damage. Conclusions The results suggest that various factors such as size, a probable different proportion of surface silanols, a balanced mechanism between calcium and magnesium cellular uptake or the different crystalline nature may have contributed to this finding; however, future research is needed to clarify the underlying mechanisms.
机译：简介表面的性质对于确定二氧化硅粉末的生物活性至关重要。在本研究中尝试了通过溶胶 - 凝胶法合成的生物活性玻璃陶瓷（BGCs）的毒性和表面性质之间的新相关性。方法评价BGCs在其对红细胞表面（RBC）的表面后的行为，并基于带3，脂质过氧化的抗磷酸酪氨酸免疫印迹，血液过氧化，产生反应性氧物种的血栓损伤，测定它们的毒性效应和抗氧化酶生产。特别地，在三个烧结温度下合成并处理了三种BGC（T1 = 835℃，T2 = 1000℃和T3 = 1100℃），以研究表面电荷或结构和溶血潜力之间的可能关系。结果它们基于溶血的毒性是剂量依赖性，而BGC-T2在与其他BGCS中相比，BGC-T2具有最佳的血液化性，其剂量低于0.125mg / ml可能损伤红细胞。另一方面，所有BGCS在某些浓度下促进了活性氧物质的生产，BGC-T2产生最低的ROS并增加RBCS中的谷胱甘肽水平保护它们的损伤。结论结果表明，尺寸等各种因素，可能不同比例的表面硅烷醇，钙和镁细胞摄取之间的平衡机制或不同的结晶性质可能有助于这种发现;但是，需要未来的研究来澄清潜在的机制。

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