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首页> 外文期刊>Cytoskeleton >Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency
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Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency

机译：对中央装置PF6蛋白内功能域的分析揭示了PF6亚复合体成员在调节鞭毛搏动频率中的作用

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摘要

Numerous studies have indicated that each of the seven projections associated with the central pair of microtubules plays a distinct role in regulating eukaryotic ciliary/flagellar motility. Mutants which lack specific projections have distinct motility phenotypes. For example, Chlamydomonas pf6 mutants lack the C1a projection and have twitchy, non-beating flagella. The C1a projection is a complex of proteins including PF6, C1a-86, C1a-34, C1a-32, C1a-18, and calmodulin. To define functional domains within PF6 and to potentially assign functions to specific C1a components, we generated deletion constructs of the PF6 gene and tested for their ability to assemble and rescue motility upon transformation of mutant pf6 cells. Our results demonstrate that domains near the carboxyl-terminus of PF6 are essential for motility and/or assembly of the projection. The amino terminal half of PF6 is not required for C1a assembly; however, this region is important for stability of the C1a-34, C1a-32, and C1a-18 sub-complex and wild-type beat frequency. Analysis of double mutants lacking the amino terminus of PF6 and outer dynein arms reveal that C1a may play a role in modulating both inner and outer dynein arm activity.
机译：大量研究表明，与中央微管对相关的七个投影中的每一个在调节真核睫毛/鞭毛运动中都起着独特的作用。缺乏特定预测的突变体具有不同的运动表型。例如，衣藻pf6突变体缺乏C1a投射，并具有抽搐，无搏动的鞭毛。 C1a投影是包括PF6，C1a-86，C1a-34，C1a-32，C1a-18和钙调蛋白的蛋白质复合物。为了定义PF6内的功能域并可能将功能分配给特定的C1a组件，我们生成了PF6基因的缺失构建体，并测试了它们在突变pf6细胞转化后组装和挽救运动性的能力。我们的结果表明，PF6羧基末端附近的域对于投影的运动性和/或组装至关重要。 C1a组装不需要PF6的氨基末端一半。但是，该区域对于C1a-34，C1a-32和C1a-18亚复合物以及野生型搏动频率的稳定性很重要。对缺少PF6氨基末端和外部达因臂的双突变体的分析表明，C1a可能在调节内部和外部达因臂的活动中发挥作用。

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