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首页> 外文期刊>Cytoskeleton >High expression of Lifeact in Arabidopsis thaliana reduces dynamic reorganization of actin filaments but does not affect plant development.
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High expression of Lifeact in Arabidopsis thaliana reduces dynamic reorganization of actin filaments but does not affect plant development.

机译：Lifeact在拟南芥中的高表达减少了肌动蛋白丝的动态重组，但不影响植物发育。

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摘要

Lifeact is a novel probe that labels actin filaments in a wide range of organisms. We compared the localization and reorganization of Lifeact:Venus-labeled actin filaments in Arabidopsis root hairs and root epidermal cells of lines that express different levels of Lifeact: Venus with that of actin filaments labeled with GFP:FABD2, a commonly used probe in plants. Unlike GFP:FABD2, Lifeact:Venus labeled the highly dynamic fine F-actin in the subapical region of tip-growing root hairs. Lifeact:Venus expression at varying levels was not observed to affect plant development. However, at expression levels comparable to those of GFP:FABD2 in a well-characterized marker line, Lifeact:Venus reduced reorganization rates of bundles of actin filaments in root epidermal cells. Reorganization rates of cytoplasmic strands, which reflect the reorganization of the actin cytoskeleton, were also reduced in these lines. Moreover, in the same line, Lifeact:Venus-decorated actin filaments were more resistant to depolymerization by latrunculin B than those in an equivalent GFP:FABD2-expressing line. In lines where Lifeact: Venus is expressed at lower levels, these effects are less prominent or even absent. We conclude that Lifeact: Venus reduces remodeling of the actin cytoskeleton in Arabidopsis in a concentration-dependent manner. Since this reduction occurs at expression levels that do not cause defects in plant development, selection of normally growing plants is not sufficient to determine optimal Lifeact expression levels. When correct expression levels of Lifeact have been determined, it is a valuable probe that labels dynamic populations of actin filaments such as fine F-actin, better than FABD2 does.
机译：Lifeact是一种新型探针，可在多种生物中标记肌动蛋白丝。我们比较了Lifeact：Venus标记的肌动蛋白丝在拟南芥根毛和表达不同水平的Lifeact：Venus的品系的根表皮细胞中的定位和重组，以及用植物常用的GFP：FABD2标记的肌动蛋白丝的定位和重组。与GFP：FABD2不同，Lifeact：Venus在尖端生长的根毛的根下区域标记了高度动态的精细F-肌动蛋白。 Lifeact：未观察到不同水平的维纳斯表达会影响植物发育。但是，在一个特征明确的标记系中，表达水平与GFP：FABD2相当，Lifeact：Venus降低了根表皮细胞中肌动蛋白丝束的重组率。在这些品系中，反映肌动蛋白细胞骨架重组的胞质链重组率也降低了。而且，在同一株系中，用Lifeact：Venus装饰的肌动蛋白丝比同等的GFP：FABD2表达株系对拉特朗菌素B的解聚反应更具抵抗力。在以较低水平表达Lifeact：Venus的行中，这些影响不太明显甚至不存在。我们得出的结论是，Lifeact：Venus以浓度依赖性方式减少拟南芥中肌动蛋白细胞骨架的重塑。由于这种减少发生在不会引起植物发育缺陷的表达水平，因此选择正常生长的植物不足以确定最佳的Lifeact表达水平。确定了Lifeact的正确表达水平后，它是一种有价值的探针，可以比FABD2更好地标记肌动蛋白丝的动态种群，例如细F-肌动蛋白。

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