Human IL-36RA production in <Emphasis Type='Italic'>Escherichia coli</Emphasis> with coexpression of <Emphasis Type='Italic'>E. coli</Emphasis> methionine aminopeptidase. II. Comparison of IL-36RA biological activity from different strains

A. A. Kolobov; E. V. Kondratyeva; T. A. Sharafutdinova; R. S. Kalinin; P. P. Nimiritsky; V. E. Stefanov; A. V. Petrov

首页> 外文期刊>Cell and Tissue Biology >Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. II. Comparison of IL-36RA biological activity from different strains

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Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. II. Comparison of IL-36RA biological activity from different strains

机译：人体IL-36ra生产中<重点类型=“斜体”>大肠杆菌具有<重点类型=“斜体”> e。 COLI 甲硫氨酸氨基肽酶。 II。不同菌株IL-36RA生物活性的比较

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AbstractProinflammatory cytokines of the interleukin-36 (IL-36) family are involved in the pathogenesis of different skin diseases in human and mice. Administration of exogenous IL-36 receptor antagonist (IL-36RA) may be an approach to therapy of different dermatitises. For its full biological activity, IL-36RA requires cleavage of N-terminal methionine residue. We created threeE. colistrains producing IL-36RA coexpressed withE. colimethionine aminopeptidase under control of different promoters. To test the biological activity of IL-36RA from different strains we transfected А549 cells with plasmid carrying the IL-36 receptor gene (IL1RL2). These cells respond to IL-36g treatment with production of IL-8, which can be quantified with ELISA. IL-36RA treatment disrupts IL-36 receptor activation by IL-36g and production of IL-8. Using this system, we proved that IL-36RA from all three producer strains is fully biologically active.]]>

机译： e。 COLI 产生IL-36ra的菌株与<重点型=“斜体”> e。 Coli 甲硫氨酸氨基肽酶的不同启动子。为了测试来自不同菌株的IL-36ra的生物活性，我们将载体载有含IL-36受体基因的质粒（<重点型=“斜体”> IL1RL2 ）。这些细胞对IL-8的产生响应IL-36G处理，其可以用ELISA量化。 IL-36ra治疗破坏IL-36G的IL-36受体激活和IL-8的产生。使用该系统，我们证明了来自所有三种生产者菌株的IL-36RA完全是生物学活性的。 ]]>

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来源
《Cell and Tissue Biology》 |2017年第6期|共5页
作者
A. A. Kolobov; E. V. Kondratyeva; T. A. Sharafutdinova; R. S. Kalinin; P. P. Nimiritsky; V. E. Stefanov; A. V. Petrov;
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作者单位

Institute of Highly Pure Biopreparations;

Institute of Highly Pure Biopreparations;

Institute of Highly Pure Biopreparations;

Institute of Highly Pure Biopreparations;

Department of Biochemistry Moscow State University;

Department of Biochemistry St. Petersburg State University;

Institute of Highly Pure Biopreparations;

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原文格式 PDF
正文语种 eng
中图分类普通生物学;
关键词
interleukin; Interleukin-36 receptor antagonist (IL-36RA); methionine aminopeptidase (MAP); recombinant protein; coexpression; methionine; А549 cells;

机译：白细胞介素;白细胞介素-36受体拮抗剂（IL-36ra）;甲硫氨酸氨基肽酶（MAP）;重组蛋白;共表达;蛋氨酸;α549细胞;

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1. Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. II. Comparison of IL-36RA biological activity from different strains [J] . A. A. Kolobov, E. V. Kondratyeva, T. A. Sharafutdinova, Cell and Tissue Biology . 2017,第6期

机译：人体IL-36ra生产中<重点类型=“斜体”>大肠杆菌具有<重点类型=“斜体”> e。 COLI 甲硫氨酸氨基肽酶。 II。不同菌株IL-36RA生物活性的比较
2. Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. I. Comparison of IL-36RA production in different strains [J] . A. A. Kolobov, E. V. Kondratyeva, T. V. Kudling, Cell and Tissue Biology . 2017,第6期

机译：人体IL-36RA在<重点型=“斜体”>大肠杆菌中的产生，具有<重点类型=“斜体”> e的共同表达> E。 COLI 甲硫氨酸氨基肽酶。 I.不同菌株中IL-36ra生产的比较
3. Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. I. Comparison of IL-36RA production in different strains [J] . A. A. Kolobov, E. V. Kondratyeva, T. V. Kudling, Cell and Tissue Biology . 2017,第6期

机译：人体IL-36ra生产中<重点类型=“斜体”>大肠杆菌具有<重点类型=“斜体”> e。 COLI 甲硫氨酸氨基肽酶。 I.在不同菌株中的IL-36ra生产的比较
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Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. II. Comparison of IL-36RA biological activity from different strains

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