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AFM Sensing Cortical Actin Cytoskeleton Destabilization During Plasma Membrane Electropermeabilization

机译:等离子体膜电透化过程中的AFM传感皮质肌动蛋白细胞骨架失稳。

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Electropermeabilization is a physical method that uses electric field pulses to deliver molecules into cells and tissues. Despite its increasing interest in clinics, little is known about plasma membrane destabilization process occurring during electropermeabilization. Having previously demonstrated the usefulness of Atomic Force Microscopy to study electropermeabilization effect on plasma membrane, we further investigated the plasma membrane destabilization process. We got mainly interested in the cytoskeleton role in stiffness of the plasma membrane, and thus in the effect of electric field on the cortical actin network. We show here that cortical actin is destabilized by electric pulses and that this effect is not directly related to the electropermeabilization of the plasma membrane. (c) 2014 Wiley Periodicals, Inc.
机译:电透化是一种物理方法,它使用电场脉冲将分子输送到细胞和组织中。尽管其在临床中的兴趣日益浓厚,但对电透化过程中发生的质膜去稳定化过程知之甚少。先前已经证明了原子力显微镜用于研究对质膜的电通透作用的有用性,我们进一步研究了质膜的去稳定过程。我们主要对细胞骨架在质膜刚度中的作用感兴趣,因此对电场对皮质肌动蛋白网络的影响也很感兴趣。我们在这里显示皮质肌动蛋白被电脉冲不稳定,并且这种作用与质膜的电透性不直接相关。 (c)2014年威利期刊有限公司

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