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首页> 外文期刊>Cartilage >Proliferation, Migration, and ECM Formation Potential of Human Annulus Fibrosus Cells Is Independent of Degeneration Status
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Proliferation, Migration, and ECM Formation Potential of Human Annulus Fibrosus Cells Is Independent of Degeneration Status

机译：人环纤维蕈类细胞的增殖，迁移和ECM形成潜力与变性状态无关

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Objective The objective was to evaluate the proliferating, migratory and extracellular matrix (ECM) forming potential of annulus fibrosus cells derived from early (edAFC) or advanced (adAFC) degenerative tissue and their usability as a possible cell source for regenerative approaches for AF closure. Design EdAFC ( n = 5 Pfirrman score of 2-3) and adAFC (n = 5 Pfirrman score of 4-5) were isolated from tissue of patients undergoing spine stabilizing surgery. Cell migration on stimulation with human serum (HS), platelet-rich plasma (PRP), and transforming growth factor β-3 (TGFB3) was assessed by migration assay and proliferation was assessed on stimulation with HS. Induction of ECM synthesis was evaluated by gene expression analysis of AF-related genes in three-dimensional scaffold cultures that have been stimulated with 5% PRP or 10 ng/mL TGFB3 and histologically by collagen type I, type II, alcian blue, and safranin-O staining. Results EdAFC and adAFC were significantly attracted by 10% HS and 5% PRP. Additionally, both cell groups proliferated under stimulation with HS. Stimulation with 10 ng/mL TGFB3 showed significant induction of gene expression of collagen type II and aggrecan, while 5% PRP decreased the expression of collagen type I. Both cell groups showed formation of AF-like ECM after stimulation with TGFB3, whereas stimulation with PRP did not. Conclusions Our study demonstrated that AF cells retain their potential for proliferation, migration, and ECM formation independent of the degeneration status of the tissue. Proliferation, migration, and ECM synthesis of the endogenous AF cells can be supported by different supplements. Hence, endogenous AF cells might be a suitable cell source for a regenerative repair approaches.
机译：目的是评价从早期（EDAFC）或先进（ADAFC）退化组织的环形纤维细胞的增殖，迁移和细胞外基质（ECM）形成潜力及其作为AF关闭的再生方法的可能性细胞来源。从接受脊柱稳定手术的患者组织中分离了eDAFC（n = 5射频得分为2-3）和adafc（n = 5 pfirrman得分4-5）。通过迁移测定评估用人血清（HS），富含血小板血浆（PRP），血小板血浆（PRP）和转化生长因子β-3（TGFB3）的细胞迁移，并评估HS刺激的增殖。通过用5％PRP或10ng / ml TGFB3刺激的三维支架培养物中的AF相关基因的基因表达分析评估ECM合成的诱导，并通过胶原蛋白I型，II型，Alcian Blue和Safranin组织学 - 染色。结果EDAFC和ADAFC显着吸引了10％HS和5％PRP。另外，两种细胞基团在用HS刺激下增殖。用10ng / ml TGFB3的刺激显示II型和蛋白的基因表达的显着诱导，而5％PRP降低了胶原蛋白类型I的表达。两种细胞基团在用TGFB3刺激后表现出AF样ECM的形成，而刺激PRP没有。结论我们的研究表明，AF细胞不与组织的退化状态无关的增殖，迁移和ECM形成的潜力。内源性AF细胞的增殖，迁移和ECM合成可以通过不同的补充剂支持。因此，内源性AF细胞可能是用于再生修复方法的合适的细胞源。

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