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首页> 外文期刊>Canadian Journal of Soil Science >Response of fungal composition and diversity to simulated nitrogen deposition and manipulation of precipitation in soils of an Inner Mongolia desert steppe of northern China
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Response of fungal composition and diversity to simulated nitrogen deposition and manipulation of precipitation in soils of an Inner Mongolia desert steppe of northern China

机译:北方内蒙古沙漠草原土壤中对氮素组成和模拟氮沉积和沉淀的响应。

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摘要

Nitrogen (N) deposition and precipitation changes can strongly influence soil microbial properties in arid and semiarid regions. Here, we examined these effects on soil samples from the Inner Mongolia desert steppe of northern China after 7 yr of consecutive simulated N deposition by adding NH4NO3 and manipulation of precipitation, using a dilution plate method, PCR analysis, and 18S rRNA sequencing. The experimental treatments were as follows: control (CK), N addition (+N), N and water addition (+N+W), and N addition plus water reduction (+N-W). In this study, 14 genera and 32 fungal species were isolated, and Penicillium was determined to be the dominant fungal group. Treatment +N-W significantly increased (by 94.8%) the number of cultivable fungi as compared with CK. Compared with the CK community, fungal communities exposed to the three treatments, especially +N+W and +N-W, showed shifts in the relative abundances of cultivable fungi. Treatment +N-W significantly enhanced species richness compared with +N at the 0-2 cm soil depth. However, N addition and manipulation of precipitation did not influence species richness, the Shannon-Weiner index, or evenness at the 0-30 cm soil depth. This study can provide insight into how fungal composition and diversity respond to climate change scenarios.
机译:氮(N)沉积和沉淀变化可以强烈影响干旱和半干旱区域的土壤微生物特性。在这里,通过添加NH 4 NO 3和操纵沉淀,通过稀释板法,PCR分析和18S rRNA测序,在连续模拟N沉积中检查了对中国北部内蒙古沙漠草原的土壤样本对土壤样本的影响。实验处理如下:对照(CK),N加(+ N),N和水另外(+ N + W),并加入加水还原(+ N-W)。在本研究中,分离了14个属和32种真菌物种,并确定了青霉素是优势真菌组。与CK相比,治疗+ N-W显着增加(通过94.8%)可种植真菌的数量。与CK群落相比,暴露于三种治疗,特别是+ N + W和+ N-W的真菌社区显示出培养真菌的相对丰富的变化。治疗+ N-W显着增强了物种丰富性,与0-2厘米土壤深度的+ N相比。然而,沉淀的N添加和操纵并没有影响物种丰富,香农 - Weiner指数,或在0-30厘米的土壤深度处的均匀度。本研究可以深入了解真菌组成和多样性如何应对气候变化情景。

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