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Viruses of stone and pome-fruit mother-tree plantations in Greece

机译:希腊的核果和石榴果母树种植园病毒

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An estimation of the virus sanitary situation of stone and pome fruit mother-tree plantations by ELISA was undertaken in 1992 and 1993 for the first time in Greece. State and private plantations were visited and samples were collected from 387 stonefruit and 376 pome fruit trees. At least one tree from each variety included in the state collections was sampled, whereas private mother tree orchards were sampled at approx. 6% of total trees. ELISA was used to test for plum pox potyvirus (PPV), prunus necrotic ringspot ilarvirus (PNRSV) and apple chlorotic leaf spot trichovirus (ACLSV) in stone fruit samples, and apple mosaic ilarvirus (ApMV), apple stem grooving capillovirus (ASGV) and ACLSV in pome fruit samples. Virus incidence greatly differed according to the location of the plantations. In the case of viruses possessing a natural means of dissemination, the most serious problems were encountered when mother trees were located near commercial orchards. PPV and PNRSV incidence in peach trees inNaoussa, Imathia (northern Greece) rose to 100 and 48%, respectively; PNRSV incidence in almond trees in Volos, Magnissia (central Greece) to 94%. Peach and almond plantations away from production areas showed lower virus incidences (ranging from 0-33%)and they could be sanitated by careful indexing and further eradication of infected trees. Isolated apricot mother tree plantations in Argolida county (southern Greece) were free of PPV, whereas in the nearby Korinthia county, the PPV incidence was 1%.ACLSC, which is only disseminated by the infected propagation material, was most prevalent in pome fruits with an incidence ranging from 29.4-100% in apple trees and from 20-27% in private pear plantations. ApMV incidence in apple trees was 2.7-10.6% andthat of ASGV, identified for the first time, was 0-14.7%.
机译:ELISA法于1992年和1993年首次在希腊对核果树和石榴果母树人工林的病毒卫生状况进行了估算。参观了国有和私有种植园,并从387个核果树和376个石榴果树中收集了样品。从国家收集的每个品种中至少采集了一棵树,而对私人母树果园的采样率大约为50。占树木总数的6%。 ELISA用于检测核果样品中的李子痘病毒(PPV),李属坏死环斑伊拉病毒(PNRSV)和苹果绿化叶斑滴虫病毒(ACLSV),以及苹果花叶病毒(ApMV),苹果茎沟病毒(ASGV)和梨果样品中的ACLSV。病毒的发生率根据种植园的位置而有很大差异。对于具有自然传播手段的病毒而言,当母树位于商业果园附近时会遇到最严重的问题。在希腊北部Imathia的Naoussa的桃树中,PPV和PNRSV的发生率分别上升至100%和48%;在Magnissia(希腊中部)的沃洛斯州,杏仁树中PNRSV的发生率为94%。远离生产区的桃子和杏仁种植园显示出较低的病毒发生率(范围为0-33%),可以通过仔细索引和进一步根除感染树木来对其进行消毒。 Argolida县(希腊南部)的孤立杏母树人工林不含PPV,而在附近的Korinthia县,PPV发生率为1%。ACLSC仅由感染的繁殖材料传播,在带有苹果树的发病率从29.4-100%不等,私人梨园的发病率从20-27%不等。苹果树中的ApMV发生率为2.7-10.6%,首次发现的ASGV的发生率为0-14.7%。

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