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首页> 外文期刊>Bulletin of the Chemical Society of Japan >Liposome-Based Biosensors Using Phytase Immobilized on Polypyrrole Films for Phytic Acid Determination
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Liposome-Based Biosensors Using Phytase Immobilized on Polypyrrole Films for Phytic Acid Determination

机译：基于脂质体的生物传感器，使用固定在聚吡咯膜上的植酸酶进行植物磷酸薄膜测定

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摘要

Phytase was immobilized onto electrochemically prepared polypyrrole (PPy) films either in the free, native form (Phy) or encapsulated in phospholipid dipalmitoyl phosphatidylglycerol (DPPG) liposomes (lipo-Phy). The incorporation of Phy into the PPy matrix was verified using cyclic voltammetry, polarization-modulated infrared reflection absorption spectroscopy, and scanning electron microscopy. PPy, PPy/Phy and PPy/lipo-Phy films were characterized by amperometric experiments in phytic acid (PA) solutions at different concentrations. PPy/lipo-Phy showed a more sensitive response, which was confirmed in stable current vs concentration and current vs time curves, and by a superior analytical performance with a saturation concentration of 3.0 mmol L-1 PA. A multidimensional projection technique, IDMAP, was used to project the voltammetric data, and confirmed the higher selectivity for PPy/lipo-Phy by distinguishing PA in a specific range of concentrations.
机译：将植酸酶固定在自由，天然形式（PHY）中以自由，天然形式（PHY）固定在电化学制备的聚吡咯（PPY）膜上或包封在磷脂二硫代磷脂酰甘氨酸（DPPG）脂质体（Lipo-PHY）中。 使用循环伏安法，偏振调制的红外反射吸收光谱和扫描电子显微镜验证PHY进入PPY矩阵。 PPY，PPY / PHY和PPY / LIPO-PHY薄膜的特征是在不同浓度下的植物酸（PA）溶液中的功率实验。 PPY / Lipo-PHY显示出更灵敏的反应，其在稳定的电流Vs浓度和电流Vs时间曲线中确认，并通过饱和浓度为3.0mmol L-1 Pa的卓越分析性能。 多维投影技术IDMAP用于投影伏安数据，并通过区分PA在特定的浓度范围内确认PPY / LIPO-PHY的选择性更高。

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