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Solid state protein NMR: major problems solved

机译:固态蛋白质NMR:解决了主要问题

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摘要

Protein NMR spectroscopy in the solid state overcomes the molecular weight limit that affects solution NMR. Up until now, however, the sensitivity of solid state NMR was poor because only ~(13)C detection was practical unless the proteins were perdeuterated, which is expensive. Furthermore, the samples had to be microcrystalline, which can be tricky to achieve for proteins.
机译:固态的蛋白质NMR光谱克服了影响溶液NMR的分子量限制。 然而,直到现在,固态NMR的敏感性差,因为〜(13)C检测实用,否则蛋白质备杂化,这是昂贵的。 此外,样品必须是微晶,这可能是棘手的,以实现蛋白质。

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