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首页> 外文期刊>ChemPlusChem >Zinc(II) Ion Sensing in Aqueous Micellar Solution Using Modified Bipyridine-Based 'Turn-On' Fluorescent Probes and its Application in Bioimaging
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Zinc(II) Ion Sensing in Aqueous Micellar Solution Using Modified Bipyridine-Based 'Turn-On' Fluorescent Probes and its Application in Bioimaging

机译：锌（II）使用改性的硼基“开启”荧光探针的含水胶束溶液中的离子感测及其在BioMimaging中的应用

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The bipyridine-based constructs 4-(pyridine-2-yl)-3H-pyrrolo[2,3-c]quinoline (PPQ) and [6-(3H-pyrrolo[2,3-c]quinolin-4-yl)-pyridin- 2-yl] methanol (PPQ-OH) and their assemblies with surfactants are evaluated as turn-on fluorescent sensors for Zn2+ ions in aqueous solution. This study strives to overcome the problem of low water solubility of the hydrophobic PPQ and PPQ-OH by using micelles. Whereas the ligands show selective sensing behavior for Zn2+ over important biological cations including Na+, K+, Ca2+, Mg2+ in anionic sodium dodecyl sulfate and non-ionic Tween 80 micelles, no Zn2+ sensing is observed in cationic cetyltrimethylammonium bromide micelles. Unlike in DMF, Cd2+ interference is observed in aqueous conditions, which can be avoided either by performing the study at pH >= 9 or by carrying out a time-resolved fluorescence study. Analysis of the Job plot data, the fluorescence lifetimes, and experiments on varying micellar shape and pH, confirms that the coordination volume of the resulting octahedral metal complex and formation of a five-membered chelate ring are critical factors for Cd2+ interference. The described sensing systems are capable of detecting Zn2+ ions at the micromolar level. Additionally, it is shown that PPQ and PPQ-OH can be used to detect Zn2+ in HeLa cells under physiological conditions in bioimaging studies.
机译：基于Bijyridine的构建体4-（吡啶-2-基）-3H-吡咯烷[2,3-C]喹啉（PPQ）和[6-（3H-吡咯罗[2,3-C]喹啉-4-基） - 吡啶-2-基]甲醇（PPQ-OH）及其具有表面活性剂的组件被评价为水溶液中的Zn2 +离子的导通荧光传感器。本研究致力于使用胶束克服疏水PPQ和PPQ-OH的低水溶性的问题。虽然配体显示出Zn2 +的选择性感测行为，但在包括Na +，K +，Ca2 +，Mg2 +中的重要生物阳离子，在阴离子十二烷基硫酸钠和非离子吐温80米芯片中，在阳离子甲甲基三甲基溴化铵胶束中观察到Zn2 +感测。与DMF中不同，在水性条件下观察到CD2 +干扰，通过在pH> = 9处进行研究或通过进行时间分辨荧光研究，可以避免。作业曲线数据的分析，荧光寿命和对不同胶束形状和pH的实验，证实所得八面体金属络合物的配位体积和五元螯合环的形成是CD2 +干扰的关键因素。所描述的传感系统能够检测微摩尔水平的Zn2 +离子。另外，显示PPQ和PPQ-OH可用于在生物分析研究中的生理条件下检测HeLa细胞中的Zn2 +。

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