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首页> 外文期刊>Acta histochemica et cytochemica >Demonstration of Epstein-Barr Virus Genome in Archival Paraffin Sections of Hodgkin's Lymphoma Autopsied by Dr. Thomas Hodgkin Nearly 170 Years ago
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Demonstration of Epstein-Barr Virus Genome in Archival Paraffin Sections of Hodgkin's Lymphoma Autopsied by Dr. Thomas Hodgkin Nearly 170 Years ago

机译：约170年前托马斯·霍奇金博士尸检的霍奇金淋巴瘤档案石蜡切片中的爱泼斯坦-巴尔病毒基因组演示

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Four unstained glass slides prepared from three cases autopsied by Dr. Thomas Hodgkin himself over 170 years ago were obtained during a visit to Gordon Museum, Guy's Hospital Medical School, London, UK. Hematoxylin and eosin staining confirmed the well-preserved spleen and autolytic liver to be massively infiltrated by Reed-Sternberg (R-S) cells in fibronodular background, and the two lymph nodes to have numerous R-S cells in one case and features of non-Hodgkin's lymphoma in the other. After bleaching the dyes, sections were transferred to silane-coated glass slides, and for the liver and spleen, the sections were divided into two. The labeled streptavidin biotinylated peroxidase method was employed for detecting CD15, CD30 and LMP-1, and the in situ hybridization technique for EBER-1 (Epstein-Barr virus-related small nuclear RNA). For the liver and spleen, EBER-1 was re-stained using sections negative for LMP-1. CD15 was detected on R-S cells in the liver. CD30 and LMP-1 were undetectable. EBER-1 was demonstrated in the nuclei of R-S cells in the lymph node, liver and spleen. The lymph node of non-Hodgkin's lymphoma was negative for EBER-1. The present study confirmed the diagnosis of Hodgkin's lymphoma and Epstein-Barr viral infection in R-S cells in archival samples from two of the three original cases autopsied by Dr. Thomas Hodgkin.
机译：在访问英国伦敦盖伊医院医学院的戈登博物馆期间，从170年前由托马斯·霍奇金博士亲自对其进行尸检的三个病例中制备了四个未染色的载玻片。苏木精和曙红染色证实，保存完好的脾脏和自溶性肝在纤维结节背景中被Reed-Sternberg（RS）细胞大量浸润，并且两个淋巴结在一种情况下具有大量RS细胞，并且在非霍奇金淋巴瘤中具有特征另一个。对染料进行漂白后，将切片转移到涂有硅烷的载玻片上，并将肝脏和脾脏的切片分为两部分。标记的链霉亲和素生物素化的过氧化物酶方法用于检测CD15，CD30和LMP-1，以及EBER-1（与爱泼斯坦-巴尔病毒相关的小核RNA）的原位杂交技术。对于肝脏和脾脏，使用LMP-1阴性切片将EBER-1重新染色。在肝脏的R-S细胞上检测到CD15。 CD30和LMP-1无法检测到。在淋巴结，肝脏和脾脏的R-S细胞核中证实了EBER-1。非霍奇金淋巴瘤的淋巴结EBER-1阴性。本研究证实了托马斯·霍奇金（Thomas Hodgkin）尸检的三例原始病例中的两例在档案样本中R-S细胞中霍奇金淋巴瘤和爱泼斯坦-巴尔病毒感染的诊断。

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