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Micropropagation of Protea repens

机译：普罗梯亚木的微繁殖

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摘要

Actively growing shoots on field-grown plants of P. repens cv. Embers were treated with fungicides or growth regulators prior to the collection of multinodal explants (3-5 nodes). The explants were established on half-strength MS medium supplementedwith 1 mg benzyladenine (BA)/litre. Treatment of mother plant shoots with 200 mg BA/litre significantly reduced browning and promoted bud sprouting in vitro. Treatment of mother plants with captafol (Difolatan, 1 g/litre) or iprodione (Rovral Flo, 1 ml/litre) reduced the contamination rate during establishment to 14%, compared with 90% in the control. Bud break in vitro was increased by the addition of gibberellic acid to the medium at 6 mg/litre. Somatic embryos developed from the base of shootlets throughout the multiplication stage.
机译：在P. repens cv的田间生长的植物上活跃地生长新芽。在收集多结外植体（3-5节）之前，先用杀菌剂或生长调节剂处理余烬。外植体在补充有1 mg苄基腺嘌呤（BA）/升的半强度MS培养基上建立。用200 mg BA / L处理母本植物的新芽可显着减少褐变并促进体外芽萌发。用captafol（Difolatan，1 g / L）或Iprodione（Rovral Flo，1 ml / L）处理母本植物，在建立过程中将污染率降低到14％，而对照组为90％。通过向培养基中添加6 mg / L的赤霉素增加体外芽裂。在整个繁殖阶段，从胚芽的基部发育出体细胞胚。

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