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首页> 外文期刊>Acta Horticulturae >Assessment of the microbial status in closed hydroponic system using phospholipid fatty acid analysis.
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Assessment of the microbial status in closed hydroponic system using phospholipid fatty acid analysis.

机译：使用磷脂脂肪酸分析评估封闭水培系统中的微生物状况。

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Phospholipid fatty acid analysis (PLFA) profiles were used to detect the changes in the microbial composition of the rhizosphere of hydroponically grown tomato plants. Root tissues were sampled from two different cultivation systems, i.e. a closed hydroponic deep flow system in a greenhouse and a small-scale climate chamber system. In the closed hydroponic system, tomato (Lycopersicon esculentum) roots were subjected to the following treatments: (i) unbacterized roots (control), (ii) single strainplant growth promoting rhizobacteria (PGPR); Pseudomonas fluorescens 5.014, and (iii) multiple strain PGPR Pseudomonas fluorescens 5.014, Xanthomonas maltophilia [Stenotrophomonas maltophilia] 18.013 and an unidentified Gram-positive isolate 19.018. In the small-scale climate chamber, plants of tomato (cultivars Gitana, Aromata, Armada and Elin), sweet pepper (Capsicum annuum cv. Evident) and lettuce (Lactuca sativa cv. Grand Rapids) were cultivated and harvested after 6 weeks; tomato cv. Gitana plantswere also harvested after 4 and 8 weeks. In addition 6-week-old tomato plants were cultivated in a climate chamber and subjected to the following treatments: (i) control, (ii) Pythium ultimum, (iii) P. ultimum and Pseudomonas fluorescens 5.014 (wild-type), and (iv) Pythium ultimum and Pseudomonas fluorescens5-2/4 (genetically modified type). PLFA profiles, isolated by split-phase extraction and step-wise lipid separation and identified by gas chromatography, revealed significant differences with respectto plant species, cultivars, plant age and treatment with genetically closely related bacterial isolates.
机译：磷脂脂肪酸分析（PLFA）谱用于检测水培番茄植株根际微生物组成的变化。根组织是从两种不同的栽培系统中取样的，即温室中的封闭水培深流系统和小型气候箱系统。在封闭的水耕系统中，对番茄（番茄根）的根进行以下处理：（i）未杀菌的根（对照），（ii）单一菌株促进根瘤菌生长（PGPR）;荧光假单胞菌5.014，和（iii）多株PGPR荧光假单胞菌5.014，麦芽黄单胞菌[嗜麦芽单胞菌] 18.013和未鉴定的革兰氏阳性分离株19.018。在小规模的气候室中，种植了番茄（栽培品种Gitana，Aromata，Armada和Elin），甜椒（Capsicum annuum cv。Evident）和生菜（Lactuca sativa cv。Grand Rapids），并在六周后收获。番茄简历在第4和第8周后也收获了Gitana植物。此外，将6周龄的番茄植株种植在气候室中，并进行以下处理：（i）对照，（ii）腐霉菌，（iii）腐霉菌和荧光假单胞菌5.014（野生型），以及（iv）腐霉菌和荧光假单胞菌5-2 / 4（基因修饰型）。通过分相萃取和逐步脂质分离分离并通过气相色谱法鉴定的PLFA谱显示，在植物种类，品种，植物年龄和基因密切相关的细菌分离株处理方面存在显着差异。

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