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首页> 外文期刊>Acta Horticulturae >Identification of a new Trichovirus in some fruit trees infected by Apple chlorotic leaf spot virus (ACLSV).
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Identification of a new Trichovirus in some fruit trees infected by Apple chlorotic leaf spot virus (ACLSV).

机译：在感染苹果绿化叶斑病毒（ACLSV）的某些果树中鉴定出新的滴虫病毒。

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ACLSV has been reported to be responsible for highly different symptomatologies in Prunus species and these various symptom types were generally thought to be related to the expression of distinct and stable ACLSV strains or to result from specific virus strain/host genotype interactions. A representative collection of stone-fruit isolates of ACLSV from southern Italy (University of Naples) and from a number of other countries (INRA of Bordeaux and Ctifl, Centre de Lanxade) was examined to study the cause of these very different symptomatologies. In an attempt to characterize severe isolates of ACLSV, a degenerate-primers nested RT-PCR technique previously developed (PDO-PCR, Foissac et al., 2001) for the polyvalent detection of viruses belonging to the Fovea-, Tricho- and Capillovirus genera was used. Surprisingly, this technique revealed the presence, in some plant accessions, of a new virus belonging to the Trichovirus genus, which was systematically found in mixed infection with ACLSV. The availability of nucleotide sequence information has allowed the design of specific nested RT-PCR primers which have been used to study the variability of the new agent, which appears to be quite large. Further work is clearly needed to understand the distribution of this new agent and its possible contribution to symptomatologies previously associated with ACLSV isolates.
机译：据报道，ACLSV是李属物种中症状差异很大的原因，这些不同的症状类型通常被认为与独特而稳定的ACLSV病毒株的表达有关，或者是由特定病毒株/宿主基因型的相互作用所致。对来自意大利南部（那不勒斯大学）和许多其他国家（波尔多和Ctifl的INRA，兰克萨德中心）的ACLSV核果分离株的代表性收集物进行了研究，以研究这些症状非常不同的原因。为了鉴定ACLSV的严重分离株，先前开发了一种简并引物嵌套式RT-PCR技术（PDO-PCR，Foissac等，2001），用于多型检测凹窝病毒，毛滴虫病毒和毛细支胞病毒属的病毒被使用了。出人意料的是，该技术揭示了在某些植物种中存在一种属于毛滴虫属的新病毒，该病毒是在ACLSV的混合感染中系统地发现的。核苷酸序列信息的可用性允许设计特定的嵌套式RT-PCR引物，这些引物已被用于研究新试剂的变异性，该变异性似乎很大。显然需要进一步的工作来了解这种新药物的分布及其对以前与ACLSV分离物有关的症状的可能贡献。

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