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首页> 外文期刊>Acta Horticulturae >Asian prunus latent virus: an unusual Potyvirus detected in germplasm from East Asia
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Asian prunus latent virus: an unusual Potyvirus detected in germplasm from East Asia

机译：亚洲李潜伏病毒：在东亚种质中发现的一种不寻常的马铃薯病毒

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摘要

Previous work has indicated that certain peach and Prunus mume germplasms from China and Japan were infected by a new Potyvirus designated Asian prunus latent virus (APLV). The virus reacted positively with some plum pox potyvirus (PPV) coat proteinantisera in ELISA, but other tests based on PCR indicated that the virus was not PPV, but possibly a different latent Potyvirus. Data showing some of the unusual features of this virus are presented. Extracts of plant leaves infected with different virus isolates tested positive for the presence of a protein of Mr 68 kD that reacted with antisera to the cylindrical inclusion (CI) protein of tobacco etch potyvirus (TEV) and maize dwarf mosaic potyvirus (MDMV) in western blots. ELISA indicated the presence of a protein that cross reacted with antisera to the CI protein of TEV and MDMV in infected pant tissue. Cytoplasmic inclusions were not detected in thin sections of infected tissue, but were detected in thin sections of PPV or TEV-infected leaves. Immunogold localization failed to reveal the presence of CI proteins in the APLV-infected tissue cells. Antisera to TEV and MDMV coat proteins failed to react with any protein in the infected samples. Only a few flexuous virus particles were found in tissue homogenates subjected to partial purification normally used for potyviruses. It is speculated that APLV is an unusual Potyvirus in that the CI protein translated from the virus genome fails to polymerize into cytoplasmic inclusions characteristic of potyviruses. It is suggested that the virus may have defects that prevent the assembly of virus particles or may have unstable particles which break up during the purification process.
机译：先前的工作表明，来自中国和日本的某些桃和李梅种质被一种名为亚洲李潜伏病毒（APLV）的新型波多病毒感染。该病毒与ELISA中的一些李子痘病毒（Peppoy potyvirus）外壳蛋白抗血清呈阳性反应，但其他基于PCR的测试表明该病毒不是PPV，可能是潜伏的马铃薯病毒。提供了显示该病毒某些异常特征的数据。感染了不同病毒分离株的植物叶片提取物的68 kD先生蛋白呈阳性反应，该蛋白与抗血清反应，与西部地区的烟草蚀刻性杯状病毒（TEV）和玉米矮化花叶病毒（MDMV）的圆柱形包涵体（CI）蛋白反应污点。 ELISA表明在感染的裤子组织中存在与抗血清交叉反应的蛋白，该蛋白与TEV和MDMV的CI蛋白。在感染组织的薄切片中未检测到细胞质内含物，但在PPV或TEV感染的叶片的薄切片中检测到了细胞质内含物。免疫金的定位未能揭示在APLV感染的组织细胞中CI蛋白的存在。 TEV和MDMV外壳蛋白的抗血清无法与被感染样品中的任何蛋白反应。在组织匀浆中仅发现了少量弯曲病毒颗粒，该匀浆经过部分纯化后常用于马铃薯病毒。据推测，APLV是一种不寻常的马铃薯病毒，因为从病毒基因组翻译的CI蛋白不能聚合成马铃薯病毒特征性的细胞质内含物。建议病毒可能具有阻止病毒颗粒聚集的缺陷，或者可能具有在纯化过程中分解的不稳定颗粒。

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