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首页> 外文期刊>Acta Horticulturae >Aphids as potential vectors of plum pox virus: comparison of methods of testing and epidemiological consequences
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Aphids as potential vectors of plum pox virus: comparison of methods of testing and epidemiological consequences

机译：蚜虫作为梅花痘病毒的潜在载体：检测方法和流行病学后果的比较

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A simple method named "unrestricted probing" was designed to determine if a given aphid species can be a vector of plum pox potyvirus (PPV). The method involved placing together in a cage (50 X 40 X 40 cm) of several thousand aphids of a given species uninfected herbaceous plants susceptible to PPV mixed with virus-infected plants. Aphid colonies were located in a dark container which was introduced into the cage. There, the aphids were free to leave their host plant, walk out of the container andmove and probe the infected and healthy plants. The "unrestricted probing" method and several other methods used to transmit PPV were compared using several aphid species. The "unrestricted probing" method appeared to be the most successful. Using this method with a large number of aphid species, new vectors of PPV were found (such as Rhopalosiphum padi and Aphis hederae) and Aphis gossypii and A. fabae were confirmed as PPV vectors. It was also found that distinct clones of A. gossypii transmit PPV with significantly different efficiencies. It is proposed that the role of different aphid species in PPV epidemiology on Prunus should be reassessed.
机译：设计了一种名为“不受限制的探测”的简单方法，以确定给定的蚜虫物种是否可以是梅花痘病毒（PPV）的载体。该方法涉及将几千只给定物种的蚜虫的笼中（50 X 40 X 40 cm）放在一起，将未感染PPV的草本植物与感染了病毒的植物混合在一起。蚜虫菌落位于一个黑暗的容器中，该容器被引入笼中。在那里，蚜虫可以自由离开寄主植物，走出容器并移动并探查感染的健康植物。使用几种蚜虫物种比较了“无限制探测”方法和其他几种用于传播PPV的方法。 “无限制探测”方法似乎是最成功的。使用该方法对大量的蚜虫种类进行检测，发现了新的PPV载体（如红景天和棉蚜），并证实了棉蚜和短吻线虫为PPV载体。还发现棉衣曲霉的不同克隆以显着不同的效率传递PPV。建议应重新评估不同蚜虫在李子PPV流行病学中的作用。

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