Detection of phytoplasmas in fruit trees by polymerase chain reaction (PCR) in Spain

Avinent L; Llacer G

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Detection of phytoplasmas in fruit trees by polymerase chain reaction (PCR) in Spain

机译：通过聚合酶链反应（PCR）在西班牙检测果树中的植物质原体

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PCR has recently been used in Spain to detect phytoplasmas associated with fruit tree diseases, such as apricot chlorotic leaf roll, apple proliferation and pear decline. For a PCR reaction, a fragment of the 165 rRNA gene was selected as template DNA and specific and non-specific primers were used. PCR amplification products were analysed by RFLP with AluI and RsaI enzymes. Preliminary results were obtained for the presence of phytoplasmas in fruit trees, as was previously described for apricot, plum and apple trees. This is the first report of the presence of phytoplasmas in pear trees in Spain.

机译：PCR最近已在西班牙用于检测与果树病相关的植物原质，如杏黄化叶卷，苹果繁殖和梨衰退。对于PCR反应，选择165rRNA基因的片段作为模板DNA，并使用特异性和非特异性引物。使用AluI和RsaI酶通过RFLP分析PCR扩增产物。如先前对杏树，李子树和苹果树所述，获得了果树中存在植物原浆的初步结果。这是西班牙梨树中存在植原体的首次报道。

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《Acta Horticulturae》 |1995年第386期|共4页
作者
Avinent L; Llacer G;
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原文格式 PDF
正文语种 eng
中图分类园艺;
关键词
plant diseases; plant pathogens; plant pathogenic bacteria; pears; detection; molecular genetics; polymerase chain reaction; techniques; fruit crops; plant pathology; Pyrus; bacteria; Spain;

机译：植物病害;植物病原体;植物病原细菌;豌豆;检测;分子遗传学;聚合酶链反应;技术;水果作物;植物病理学;梨属;细菌;西班牙;

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1. Detection of phytoplasmas in fruit trees by polymerase chain reaction (PCR) in Spain [J] . Avinent L, Llacer G Acta Horticulturae . 1995,第386期

机译：通过聚合酶链反应（PCR）在西班牙检测果树中的植物质原体
2. Single-Step Multiplex Reverse Transcription-Polymerase Chain Reaction (m-RT-PCR) for Simultaneous Detection of Five RNA Viruses Affecting Stone Fruit Trees [J] . Sahar A. Youssef, A.A. Shalaby International Journal of Virology . 2009,第2期

机译：单步多重逆转录聚合酶链反应（m-RT-PCR）同时检测五种影响核果树的RNA病毒
3. Single-Step Multiplex Reverse Transcription-Polymerase Chain Reaction (m-RT-PCR) for Simultaneous Detection of Five RNA Viruses Affecting Stone Fruit Trees [J] . Sahar A. Youssef, A.A. Shalaby International Journal of Virology . 2009,第2期

机译：单步多重逆转录聚合酶链反应（m-RT-PCR）同时检测五种影响核果树的RNA病毒
4. Detection of Verocytotoxygenic Escherichia coli (VTEC) from Brilliant Green Lactose Bilebroth (BGLB) by Polymerase Chain Reaction (PCR) [C] . Yatri Drastini, Rini Widayanti, Winda Ayuningtyas International Conference of the International Society for Economics and Social Science on Animal Health Southeast Asia . 2019

机译：通过聚合酶链式反应（PCR）从辉煌的绿色乳糖胆管（BGLB）中检测比菌毒素大肠杆菌（VTEC）（PCR）
5. Rapid detection of “Norwalk-like viruses” by reverse transcription-polymerase chain reaction (RT-PCR) and southern blot hybridisation (SBH) in outbreaks of acute gastroenteritis in eastern Ontario, Canada [D] . Wires, Shannon Meredith 2001

机译：通过逆转录聚合酶链反应（RT-PCR）和Southern blot杂交（SBH）快速检测加拿大安大略省东部急性肠胃炎暴发中的“ Norwalk样病毒”
6. Analytical Performance of Four Polymerase Chain Reaction (PCR) and Real Time PCR (qPCR) Assays for the Detection of Six Leishmania Species DNA in Colombia [O] . Cielo M. León, Marina Muñoz, Carolina Hernández, -1

机译：四种聚合酶链反应（PCR）和实时荧光定量PCR（qPCR）检测哥伦比亚六种利什曼原虫DNA的分析性能
7. PCR(Polymerase Chain Reaction) Detection of Phytoplasma in Phloem Sap Collected by Laser Stylectomy. [O] . Mamoru SATO, Shigetou NAMBA, Maki KATSUHARA, 1996

机译：通过激光型矫形术收集的PCR（聚合酶链反应）检测Phloem SAP中的植物。

Detection of phytoplasmas in fruit trees by polymerase chain reaction (PCR) in Spain

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