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首页> 外文期刊>Acta Horticulturae >Micropropagation of Rehmannia glutinosa as medicinal plant by shoot tip and root segment culture
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Micropropagation of Rehmannia glutinosa as medicinal plant by shoot tip and root segment culture

机译：芽尖和根段培养物微生地黄为药用植物

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摘要

Root tubers of R. glutinosa are used in traditional medicine to stop bleeding, to promote the production of body fluids and to cure dizziness, palpitations and insomnia. Methods for the in vitro propagation of R. glutinosa cv. Danyang were investigated. Cultures from root segments were unsuccessful due to systemic internal contamination of roots; the use of 4 antibiotics failed to control this contamination. Shoot proliferation from apical meristems was observed on MS medium supplemented with BA (1-5 mg/litre), IAA (0.3 mg/litre) and 3% sucrose. The addition of 0.1-0.3% activated charcoal increased shoot growth, promoted root initiation and growth, reduced the incidence of vitrification but inhibited shoot proliferation. The addition of growth inhibitors (ancymidol, ABA, chlormequat or PBZ) had no effect on preventing vitrification or reducing shoot growth or shoot proliferation. Rooting in vivo from shoots was promoted by soaking the base of shoots in IBA at 100 mg/litre for 15-60 min. or treating with 0.1% IBA rooting powder prior to planting in 1:1 vermiculite:perlite mixture.
机译：在传统医学中，R.glutinosa的块根块茎可以止血，促进体液的产生并治疗头晕，心和失眠。 R.glutinosa cv。的体外繁殖方法对丹阳进行了调查。根段的培养由于根的系统内部内部污染而失败。使用4种抗生素未能控制这种污染。在补充了BA（1-5 mg / L），IAA（0.3 mg / L）和3％蔗糖的MS培养基上观察到根尖分生组织的芽增殖。添加0.1-0.3％的活性炭可增加枝条的生长，促进根的萌生和生长，减少玻璃化的发生率，但抑制枝条的增殖。添加生长抑制剂（兰定醇，ABA，氯麦草或PBZ）对防止玻璃化或减少枝条生长或枝条增殖没有影响。将芽的基部以100 mg / L的浓度浸入IBA中15-60分钟，可促进从芽中生根。或在种植于1：1 ver石：珍珠岩混合物中之前用0.1％IBA生根粉处理。

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