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首页> 外文期刊>Acta Horticulturae >Genetics and complementation of DNA regions involved in amylovoran synthesis of Erwinia amylovora and stewartan synthesis of Erwinia stewartii
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Genetics and complementation of DNA regions involved in amylovoran synthesis of Erwinia amylovora and stewartan synthesis of Erwinia stewartii

机译：链淀粉欧文氏菌合成淀粉和斯图尔坦合成欧文氏菌的DNA区域的遗传和互补

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摘要

E. amylovora and E. stewartii form similar exopolysaccharide (EPS) capsules, which are the products of the ams and cps gene clusters, respectively. Nucleotide sequence analysis has indicated that these DNA regions share many common functions. Mutants in ams and cps genes are nonpathogenic and can also be heterologously complemented by EPS genes from the other species. Depending on the chromosomal mutation, restoration of EPS synthesis in the foreign host requires a set of cloned ams or cps genes. The nature of the EPS produced by the merodiploids was determined by degradation with a bacteriophage EPS-depolymerase specific for stewartan, binding to an amylovoran-specific lectin, compositional analysis and methylation analysis. The ams mutants complemented with the cps cluster produced stewartan and lost virulence on pear slices. The cps mutants complemented with the ams cluster produced amylovoran and regained virulence to maize seedlings. An exception was a complemented cpsK mutant that producedstewartan. These results suggest a requirement for specific sugar residues in capsules of E. amylovora in order to escape recognition by plant defences.
机译：链球菌和stewartii菌形成类似的胞外多糖（EPS）胶囊，分别是ams和cps基因簇的产物。核苷酸序列分析表明这些DNA区域具有许多共同的功能。 ams和cps基因的突变体是非致病性的，也可以与其他物种的EPS基因进行异源互补。根据染色体突变，在外源宿主中EPS合成的恢复需要一组克隆的ams或cps基因。由甲倍体产生的EPS的性质是通过用对Stewartan特异的噬菌体EPS解聚酶降解，结合到戊基戊酸酯特异的凝集素上，组成分析和甲基化分析来确定的。与cps簇互补的ams突变体在梨片上产生了Stewartan并丧失了毒力。与ams簇互补的cps突变体产生了amylovoran，并恢复了对玉米幼苗的毒力。一个例外是产生stewartan的互补cpsK突变体。这些结果表明需要一种淀粉链球菌胶囊中的特定糖残基，以逃避植物防御的识别。

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