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首页> 外文期刊>Acta Horticulturae >3' Non-coding region RT-PCR detection and molecular hybridization of plum pox virus in anthers of infected stone fruit
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3' Non-coding region RT-PCR detection and molecular hybridization of plum pox virus in anthers of infected stone fruit

机译：感染核果的花药中李子痘病毒的3'非编码区RT-PCR检测及分子杂交

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摘要

The international movement of germplasm may require movement of stone fruit anthers for germplasm collection, breeding programmes, or other purposes. To investigate the feasibility of dissemination of plum pox potyvirus (PPV) through the exchange ofPrunus anthers collected from PPV-infected trees, over 120 trees of various peach, plum and apricot cultivars were assayed in a PPV-infected experimental orchard in Cegled, Hungary. Individual trees had been examined for PPV infection by visual symptomson leaves or fruit, and/or indirect ELISA. PPV detection using RT-PCR from desiccated anthers after storage at 4℃ for 1-2 years was evaluated. Anther tissues were prepared with Gene ReleaserTM polymeric matrix, and RT-PCR assayed using DNA primers forthe 3' non-coding region (NCR) of PPV. PAGE and dot-blot hybridization of the amplified PPV product from anthers using a 3' NCR digoxigenin-labelled PPV-D cRNA probe showed high levels of hybridization signal. It is concluded that stone fruit anthers could be a source of PPV dissemination during international movement of Prunus germplasm.
机译：种质的国际转移可能需要移动核果花药以进行种质收集，育种计划或其他目的。为了研究通过交换从PPV感染的树中收集的李花药传播李子痘病毒的可能性，在匈牙利Cegled的PPV感染的试验果园中，对120多种桃，李和杏品种的树进行了分析。已经通过叶片或果实上的视觉症状和/或间接ELISA检查了单棵树的PPV感染。在4℃下保存1-2年后，使用RT-PCR从干燥的花药中检测PPV。用Gene ReleaserTM聚合基质制备花药组织，并使用DNA引物对PPV的3'非编码区（NCR）进行RT-PCR分析。使用3'NCR洋地黄毒苷标记的PPV-D cRNA探针从花药中扩增的PPV产物的PAGE和点印迹杂交显示了高水平的杂交信号。结论是核果花药可能是李种质国际转移过程中PPV传播的来源。

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