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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >NK cell compartment in the peripheral blood and spleen in adult patients with primary immune thrombocytopenia
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NK cell compartment in the peripheral blood and spleen in adult patients with primary immune thrombocytopenia

机译：NK细胞室在患有原发性免疫血小板减少症的成人患者外周血和脾脏

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Immune thrombocytopenic purpura (ITP) is a disease characterized by antibody-mediated platelet destruction. The T- and B-cell subsets have been extensively studied in primary ITP, but the NK cell compartment has been less thoroughly explored. We investigated the NK cell receptor repertoire and the functionality of NK cells in the peripheral blood and spleen in patients with primary ITP. An immunophenotypic analysis of peripheral blood lymphocytes from patients revealed that the numbers of CD19(+) B lymphocytes, CD4(+) and CD8(+) T lymphocytes and CD3(-)CD56(+) NK cells were within the normal range. No major alteration to the expression of distinct inhibitory or activating NK cell receptors was observed. The functionality of NK cells, as evaluated by their ability to degranulate in conditions of natural cytotoxicity or antibody-dependent cell cytotoxicity (ADCC), was preserved in these patients. By contrast, these stimuli induced lower levels of IFN gamma production by the NK cells of ITP patients than by those of healthy controls. We then compared the splenic NK cell functions of ITP patients with those of cadaveric heart-beating donors (CHBD) as controls. The splenic NK cells of ITP patients tended to be less efficient in natural cytotoxicity conditions and more efficient in ADCC conditions than control splenic NK cells. Finally, we found that infusions of intravenous immunoglobulin led to the inhibition of NK cell activation through the modulation of the interface between target cells and NK cells. (C) 2015 Published by Elsevier Inc.
机译：免疫血小板减少紫癜（ITP）是一种以抗体介导的血小板破坏为特征的疾病。在主要ITP中已经广泛研究了T-和B细胞子集，但是NK细胞盒已经不太彻底探索。我们研究了患有主要ITP患者外周血和脾脏中NK细胞的NK细胞受体曲目和NK细胞的功能。来自患者外周血淋巴细胞的免疫型分析显示CD19（+）B淋巴细胞，CD4（+）和CD8（+）T淋巴细胞和CD3（ - ）CD56（+）NK细胞的数量在正常范围内。没有观察到不同抑制或激活NK细胞受体的表达的重大改变。通过它们在天然细胞毒性或抗体依赖性细胞细胞毒性（ADCC）的条件下，在这些患者中进行了抑制的能力的官能团。相比之下，这些刺激诱导ITP患者NK细胞的IFNγ产生的较低水平，而不是健康对照。然后，将ITP患者的脾脏NK细胞功能与尸体心脏搏动供体（CHBD）的脾细胞功能进行比较。 ITP患者的脾细胞在天然细胞毒性条件下倾向于效率低，并且在ADCC条件下比控制脾细胞更有效。最后，我们发现通过调节靶细胞和NK细胞之间的界面，静脉内免疫球蛋白的输注导致NK细胞活化。 （c）2015年由elsevier公司发布

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    Ebbo M.; Audonnet S.; Grados A.; Benarous L.; Mahevas M.; Godeau B.; Viallard J. F.; Piperoglou C.; Cognet C.; Farnarier C.; Harle J. R.; Schleinitz N.; Vely F.;

  • 作者单位

    Aix Marseille Univ Hosp Timone Adulte AP HM Dept Med Interne F-13005 Marseille France;

    Aix Marseille Univ Hop Concept AP HM Immunol F-13005 Marseille France;

    Aix Marseille Univ Hosp Timone Adulte AP HM Dept Med Interne F-13005 Marseille France;

    Aix Marseille Univ Hosp Timone Adulte AP HM Dept Med Interne F-13005 Marseille France;

    Univ Paris Est Creteil Henri Mondor Univ Hosp AP HP Dept Internal Med Natl Referral Ctr Adult;

    Univ Paris Est Creteil Henri Mondor Univ Hosp AP HP Dept Internal Med Natl Referral Ctr Adult;

    Hop Haut Leveque CHU Bordeaux GH Sud Dept Med Interne 1 Ave Magellan F-33604 Pessac France;

    Aix Marseille Univ Hop Concept AP HM Immunol F-13005 Marseille France;

    Aix Marseille Univ Hop Concept AP HM Immunol F-13005 Marseille France;

    Aix Marseille Univ Hop Concept AP HM Immunol F-13005 Marseille France;

    Aix Marseille Univ Hosp Timone Adulte AP HM Dept Med Interne F-13005 Marseille France;

    Aix Marseille Univ Hosp Timone Adulte AP HM Dept Med Interne F-13005 Marseille France;

    Aix Marseille Univ UM2 Ctr Immunol Marseille Luminy INSERM U1104 CNRS UMR7280 F-13288 Marseille;

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  • 中图分类 医学免疫学;
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