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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Longitudinal relationships between rheumatoid factor and cytokine expression by immunostimulated peripheral blood lymphocytes from patients with rheumatoid arthritis: New insights into B-cell activation
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Longitudinal relationships between rheumatoid factor and cytokine expression by immunostimulated peripheral blood lymphocytes from patients with rheumatoid arthritis: New insights into B-cell activation

机译：类风湿性关节炎患者免疫刺激周围血液淋巴细胞与类风湿因子与细胞因子表达的纵向关系：对B细胞激活的新见解

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摘要

To identify associations between immunostimulated cytokine production and disease characteristics, peripheral blood lymphocytes were collected from 155 adult patients with rheumatoid arthritis (RA) before and after a 5-year interval. The lymphocytes were activated in vitro with T-cell stimulants, cytosine-phosphate-guanine (CpG) oligonucleotide, and medium alone (negative control). Expression of 17 cytokines was evaluated with immunoassays, and factor analysis was used to reduce data complexity and identify cytokine combinations indicative of cell types preferentially activated by each immunostimulant. The findings showed that the highest numbers of correlations were between cytokine levels and rheumatoid factor (RF) positivity and between cytokine levels and disease duration. Scores for cytokines driven by CpG and medium alone were negatively associated with RF positivity and disease duration at baseline but positively associated with both at 5 years. Our findings suggest that RF expression sustained over time increases activation of B cells and monocytes without requirements for T-cell functions.
机译：为了鉴定免疫刺激细胞因子产生和疾病特征之间的关联，在5年前和5年间隔之前和之后，从155名成年患者收集外周血淋巴细胞。用T细胞兴奋剂，胞嘧啶 - 磷酸 - 鸟嘌呤（CpG）寡核苷酸和培养基（阴性对照）体外活化淋巴细胞。用免疫测定评估17个细胞因子的表达，并且使用因子分析来减少数据复杂性，并鉴定指示每种免疫刺激剂优先激活的细胞类型的细胞因子组合。结果表明，多数相关性在细胞因子水平和类风湿因子（RF）积极之间以及细胞因子水平和疾病持续时间之间。仅由CpG和培养基驱动的细胞因子的分数与基线的RF积极性和疾病持续时间产生负面，但在5年内与两者呈正相关。我们的研究结果表明RF表达随时间持续增加，增加了B细胞和单核细胞的活化，而不要求T细胞功能。

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