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首页> 外文期刊>Acta Horticulturae >Isoenzyme gene marker in asparagus used for identification of doubled-haploids and hybrids
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Isoenzyme gene marker in asparagus used for identification of doubled-haploids and hybrids

机译：芦笋中的同工酶基因标记，用于鉴定双单倍体和杂种

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摘要

Extracts from phylloclads of 30 populations and hybrids of asparagus were analysed by starch gel electrophoresis for isoenzyme polymorphism. Six enzyme systems were examined, three of which, malate dehydrogenase (MDH), phosphoglucomutase (PGM) and shikimate dehydrogenase (SKD), showed inter- and intra-cultivar polymorphism as well as clear differences in the segregation of the hybrids studied. SKD and MDH isoenzymes identified haploids and doubled-haploids from anther culture in 2 genotypes heterozygous for both isoenzymes. Segregation of polymorphic loci was investigated in 18 pairs of male and female crosses for MDH, PGM and SKD. Models describing the Mendelian inheritance in MDH, PGM and SKD are proposed. Nine polymorphic bands in MDH, 5 in SKDand 9 in PGM are described. The Sartaguda population showed a specific band for PGM. Morado de Huetor (a locally cultivated poulation) and Triguero (wild) showed specific bands for SKD and PGM. Triguero lacked the fixed band of MDH always present in A.officinalis. Correlations occurred between the band nearest the origin for PGM and the slow band for SKD. There was an association between 2 bands of MDH and female plants.
机译：通过淀粉凝胶电泳分析了30个种群的叶甲提取物和芦笋杂种的同工酶多态性。检查了六个酶系统，其中苹果酸脱氢酶（MDH），磷酸葡萄糖变位酶（PGM）和sh草酸脱氢酶（SKD）中的三个显示了品种间和品种间多态性，并且在所研究的杂种分离方面存在明显差异。 SKD和MDH同工酶从花药培养物中鉴定出两种同工酶杂合的2种基因型的单倍体和双倍体单倍体。在MDH，PGM和SKD的18对雌雄杂交中研究了多态性基因座的分离。提出了描述MDH，PGM和SKD中孟德尔遗传的模型。描述了MDH中的9个多态带，SKD中的5个和PGM中的9个。 Sartaguda种群显示出一个特定的PGM带。 Morado de Huetor（本地种植的种群）和Triguero（野生）显示了SKD和PGM的特定波段。 Triguero缺乏A.officinalis中始终存在的MDH固定带。 PGM最接近原点的波段与SKD的慢波段之间存在相关性。 MDH的两个带与雌性植物之间存在关联。

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