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首页> 外文期刊>Acta Horticulturae >Phloroglucinol and tryptone enhance in vitro rooting and survival rate of asparagus nodal sections
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Phloroglucinol and tryptone enhance in vitro rooting and survival rate of asparagus nodal sections

机译：间苯三酚和胰蛋白enhance可提高芦笋节切片的生根和存活率

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摘要

Rooting percentage of Asparagus cv. Tainan Selection 3 (TS 3) nodal sections in MS medium with NAA, kinetin and 3% sucrose was previously found to be very low (1.7%). In this study, tryptone (250 mg/litre), phloroglucinol (PG; 162 mg/litre) and ancymidol (1.28 mg/litre) were added to a modified MS medium containing NAA (0.15 mg/litre), kinetin (0.15 mg/litre) and 6% sucrose to investigate their potential for increased in vitro rooting of TS 3. The rooting percentage in control medium was 18%, while in media with tryptone, PG or ancymidol, the rooting percentages increased to 41, 59 and 63%, respectively. With a combination of tryptone or PG with ancymidol, in vitro rooting reached 73-78%. In addition to increasing the frequency of rooting, the presence of PG or tryptone significantly increased shoot number and root length. In contrast, ancymidol reduced shoot length, but significantly increased the diameter of shoots and roots and number of shoots per plantlet. Survival rate oftransplanted plantlets was influenced by temperature, covering with a plastic bag and inoculation with Glomus spp. to induce vesicular-arbuscular mycorrhizal associations.
机译：芦笋的生根率。先前发现，在含有NAA，激动素和3％蔗糖的MS培养基中，台南选择 3（TS 3）淋巴结节非常低（1.7％）。在这项研究中，将胰蛋白（（250毫克/升），间苯三酚（PG; 162毫克/升）和兰西多（1.28毫克/升）添加到含有NAA（0.15毫克/升），激动素（0.15毫克/升）的改良MS培养基中升）和6％的蔗糖含量，以研究它们对TS 3的体外生根增加的潜力。对照培养基中的生根百分比为18％，而在含有胰蛋白,、 PG或ancymidol的培养基中，生根百分比分别提高至41％，59％和63％。将胰蛋白or或PG与ancymidol结合使用，体外生根达到73-78％。除增加生根频率外，PG或胰蛋白the的存在还显着增加了芽数和根长。相比之下，阿西咪醇减少了芽的长度，但显着增加了芽和根的直径以及每株幼苗的芽数。温度，覆盖塑料袋和接种Glomus spp会影响移植苗的成活率。诱导水泡-丛枝菌根协会。

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