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首页> 外文期刊>Acta Horticulturae >Chloroplast DNA variation in sour cherry
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Chloroplast DNA variation in sour cherry

机译：酸樱桃中的叶绿体DNA变化

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摘要

Restriction fragments of chloroplast DNA (ctDNA) from 29 sweet (Prunus avium), sour (P. cerasus), and ground (P. fruticosa) cherry selections and species hybrids were analysed to determine the maternal origin of the tetraploid sour cherry. Total leaf DNA was isolated and digested with two restriction endonucleases that preferentially cut ctDNA. The DNA fragments were then separated on agarose gels and filter-hybridized with two PstI fragments of tomato ctDNA. RFLPs were identified that differentiated the ctDNA of sweet and ground cherry. Of the 20 sour cherry selections, 17 had the ctDNA RFLPs of ground cherry while 3 had the ctDNA RFLPs of sweet cherry. These results indicate that the allotetraploid sour cherry arose independently at least twiceand that the more common maternal species is ground cherry.
机译：分析了来自29个甜樱桃（Prunus avium），酸樱桃（P. cerasus）和地面（P. fruticosa）樱桃选择和物种杂交的叶绿体DNA（ctDNA）的限制性片段，以确定四倍体酸樱桃的母体来源。分离总叶DNA并用优先切割ctDNA的两种限制性核酸内切酶消化。然后将DNA片段在琼脂糖凝胶上分离，并与番茄ctDNA的两个PstI片段过滤杂交。鉴定了RFLP，其区分了甜樱桃和地面樱桃的ctDNA。在20种酸樱桃中，有17种具有地面樱桃的ctDNA RFLP，而3种具有甜樱桃的ctDNA RFLP。这些结果表明，同种四倍体酸樱桃至少独立地出现了两次，并且更常见的母本是地面樱桃。

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