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首页> 外文期刊>Acta Horticulturae >Application of a new micropropagation system involving induction of bud clusters and somatic embryogenesis in asparagus
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Application of a new micropropagation system involving induction of bud clusters and somatic embryogenesis in asparagus

机译：涉及芽簇诱导和体细胞胚发生的新型微繁系统的应用

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A micropropagation system involving induction of multiple bud clusters and somatic embryogenesis, previously established in asparagus cv. Hiroshimagreen (2n = 30), was applied to 14 other genotypes. Bud clusters were induced from shoot apices in MSliquid medium supplemented with 10 mg/litre ancymidol using gyrating drum culture. Bud clusters were induced and subcultured in the same medium at monthly intervals from all of the tested genotypes. During each subculture they rapidly increased to 9-21 times their initial fresh weight without callus formation. Cryopreservation was successful for bud clusters in many of the genotypes. Induction of embryogenic calluses (EC) from bud clusters was examined using MS solid medium supplemented with 10-5 M 2,4-D. In genotypes with good root formation ability, EC were difficult to induce and subculture. Direct formation of globular embryos was frequently observed on bud clusters of all genotypes, and EC were formed from embryos after transfer to MS solid mediumsupplemented with 10-5 M 2,4-D. These calluses were subcultured and stable embryogenic cell lines (without non-embryogenic calluses) were obtained in 10 genotypes. Somatic embryos were formed on MS medium without growth regulators, from which plants were regenerated. It is concluded that multiple bud clusters can be used for the preservation of genetic resources, the propagation of elite clones and the initiation of somatic embryogenesis.
机译：一种微繁殖系统，涉及多个芽簇的诱导和体细胞胚发生，先前在芦笋简历中建立。广岛绿（2n = 30）被应用于其他14个基因型。使用旋转鼓式培养从补充有10 mg / L氨苄醇的MS液体培养基中的茎尖诱导芽簇。从所有测试的基因型中以每月间隔诱导芽簇并在相同培养基中继代培养。在每次亚培养期间，它们迅速增加至其初始鲜重的9-21倍，而没有愈伤组织形成。对于许多基因型的芽簇，冷冻保存都是成功的。使用补充了10-5 M 2,4-D的MS固体培养基检查了芽簇中胚性愈伤组织（EC）的诱导。在具有良好根形成能力的基因型中，EC难以诱导和继代培养。在所有基因型的芽簇上经常观察到球状胚胎的直接形成，EC转移到补充了10-5 M 2,4-D的MS固体培养基后，从胚胎中形成。将这些愈伤组织进行继代培养，并获得了10个基因型的稳定胚发生细胞系（无非胚发生愈伤组织）。在没有生长调节剂的MS培养基上形成体细胞胚，从中再生出植物。结论是，多个芽簇可用于遗传资源的保存，优良克隆的繁殖和体细胞胚发生的启动。

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