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首页> 外文期刊>Acta Horticulturae >Distribution of apple chlorotic leaf spot virus in apple shoots cultivated in vitro
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Distribution of apple chlorotic leaf spot virus in apple shoots cultivated in vitro

机译：苹果绿化叶斑病病毒在离体栽培苹果芽中的分布

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摘要

An immuno tissue-printing protocol was developed for immunological detection of apple chlorotic leaf spot trichovirus (ACLSV) coat protein within in vitro shoots of Malus. Different apple varieties showed a typical distribution pattern for ACLSV invitro. Accumulation of ACLSV was highest in the basal part of the stem and decreased towards the apex of shoots. ACLSV reached its max. level in enlarged bases of shoots, where it was unevenly distributed, as tissue was less regularly organized. In crosssections of stem tissue, virus coat protein was found within the epidermis, the cortical parenchyma and within the vascular bundles. Within pith tissue, virus protein was only detected in adjacent parts of enlarged bases when shoots were heavily infected. The degree of infection influenced the virus localization within a cross section and its spread into the meristem. Meristems of poorly infected shoots did not show visible signs of the virus.
机译：开发了一种免疫组织打印方案，用于对苹果属离体芽中的苹果褪绿叶斑滴虫病毒（ACLSV）外壳蛋白进行免疫学检测。不同的苹果品种显示了ACLSV体外的典型分布模式。 ACLSV的积累在茎的基部最高，向茎尖下降。 ACLSV达到最大值。在不规则分布的组织中，不规则分布的芽的扩大基部中的水平。在茎组织的横截面中，在表皮，皮层薄壁组织和血管束中发现了病毒外壳蛋白。在髓组织中，仅当芽被严重感染时，才在扩大的碱基的相邻部分中检测到病毒蛋白。感染的程度影响了病毒在横截面中的定位及其在分生组织中的传播。感染不良的芽的分生组织未显示出该病毒的明显迹象。

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