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首页> 外文期刊>Biosystems Engineering >Detecting maize inoculated with toxigenic and atoxigenic fungal strains with fluorescence hyperspectral imagery.
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Detecting maize inoculated with toxigenic and atoxigenic fungal strains with fluorescence hyperspectral imagery.

机译：使用荧光高光谱成像技术检测玉米中的产毒真菌和产毒真菌菌株。

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Naturally occurring Aspergillus flavus strains can be either toxigenic or atoxigenic, indicating their ability to produce aflatoxin or not. The objective was to assess, with the use of a hyperspectral sensor, the difference in fluorescence emission between maize kernels inoculated with toxigenic and atoxigenic inoculums of A. flavus. Maize ears were inoculated with AF13, a toxigenic strain of A. flavus, and AF38, an atoxigenic strain of A. flavus, at dough stage of development and harvested 8 weeks after inoculation. After harvest, single kernels were divided into three groups prior to imaging: control, adjacent, and glowing. Both sides of the kernel, germ and endosperm, were imaged separately using a fluorescence hyperspectral imaging system. After imaging each single kernel was processed with affinity column fluorimetry to determine aflatoxin level. Results from discriminant analysis of the imaging data found that the classification accuracies of the three visually designated groups were not promising. The separation of maize kernels based on different fungal inoculums yielded better results. The best results were achieved with the germ side of the maize kernels. The kernels were later grouped into 'contaminated' and 'healthy' with 20 ppb and 100 ppb thresholds. The contaminated kernels all had longer peak wavelength than did the healthy ones. Results from the discriminant analysis classification indicated overall higher classification accuracy for the 100 ppb threshold on the germ side (94.4%). The germ side was also more useful at discriminating healthy from contaminated kernels for the 20 ppb threshold
机译：天然存在的黄曲霉菌株可以是产毒的或产毒的，表明它们产生黄曲霉毒素的能力。目的是使用高光谱传感器评估黄曲霉的产毒和产毒接种物接种的玉米粒之间荧光发射的差异。在生面团的发育阶段，将玉米穗用黄曲霉的产毒菌株AF13和黄曲霉的产毒菌株AF38接种，并在接种后8周收获。收获后，将单个籽粒在成像之前分为三组：对照，相邻和发光。使用荧光高光谱成像系统分别对籽粒的两面，胚芽和胚乳进行成像。成像后，用亲和柱荧光法处理每个单核，以确定黄曲霉毒素水平。对成像数据进行判别分析的结果发现，三个视觉指定组的分类准确性不理想。基于不同真菌接种量的玉米粒分离效果更好。在玉米粒的胚芽侧获得了最佳结果。后来将这些籽粒分为20 ppb和100 ppb阈值的“污染”和“健康”。被污染的谷粒的峰值波长都比健康的谷粒更长。判别分析分类的结果表明，对于细菌一侧的100 ppb阈值，总体分类准确度更高（94.4％）。在将健康与已污染的谷粒区分开20 ppb阈值时，细菌方面也更有用

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