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首页> 外文期刊>Acta Horticulturae >Studies on several strains of apple chlorotic leaf spot virus (ACLSV) isolated from different fruit tree species
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Studies on several strains of apple chlorotic leaf spot virus (ACLSV) isolated from different fruit tree species

机译：不同果树种苹果绿化叶斑病毒（ACLSV）几种菌株的研究

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Isolation and characterization of apple chlorotic leaf spot trichovirus (ACLSV) strains occurring in fruit trees in Poland, preparation of antisera and elaborating methods for routine ACLSV diagnosis in fruit trees were studied. ACLSV was detected in apple, pear, sweet cherry, plum and quince, using indirect F(ab')2 ELISA. Six isolates of ACLSV obtained from apple, pear, plum and sweet cherry were studied and antisera were prepared against 5. Plum isolate SX/2 showed a significantly weaker reactionin ELISA with 2 antisera (prepared against apple and cherry isolates) than the other isolates. Isolate JPom, obtained from apple, reacted better with monoclonal antibodies EMA32 than the other isolates. The coat protein (CP) of the SX/2 isolate migratedfaster in SDS-PAGE than CP of other isolates tested. RT-PCR was applied to the isolates using samples of herbaceous and woody hosts. Specific products were obtained for all the isolates, except SX/2, and digested with 3 restriction enzymes. Two different patterns were found for 4 isolates.
机译：研究了波兰果树中出现的苹果绿萎病斑点滴虫病毒（ACLSV）菌株的分离和鉴定，抗血清的制备以及果树常规ACLSV诊断的详细方法。使用间接F（ab'）2 ELISA在苹果，梨，甜樱桃，李子和木瓜中检测到ACLSV。研究了从苹果，梨，李子和甜樱桃中获得的6种ACLSV分离物，并制备了针对5种抗血清。与其他分离物相比，李子分离物SX / 2在ELISA中与2种抗血清（针对苹果和樱桃分离物制备）的反应明显弱。从苹果获得的分离株JPom与单克隆抗体EMA32的反应优于其他分离株。 SX / 2分离株的外壳蛋白（CP）在SDS-PAGE中的迁移速度比测试的其他分离株的CP更快。使用草木和木本宿主样品对分离株进行RT-PCR。获得了除SX / 2以外的所有分离物的特异性产物，并用3种限制酶消化。发现了4个分离株的两种不同模式。

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