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首页> 外文期刊>Comparative biochemistry and physiology, Part D. Genomics & proteomics >RNA-Seq analysis of the bioluminescent and non-bioluminescent species of Elateridae (Coleoptera): Comparison to others photogenic and non-photogenic tissues of Elateroidea species
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RNA-Seq analysis of the bioluminescent and non-bioluminescent species of Elateridae (Coleoptera): Comparison to others photogenic and non-photogenic tissues of Elateroidea species

机译：Elateridae（鞘翅目）的生物发光和非生物发光物种的RNA-SEQ分析：与Elateroidea物种的其他初探和非上光组织的比较

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摘要

Bioluminescence in the superfamily Elateroidea is produced by specific light organs distributed in different parts of the body depending on the life stage and family. Whereas the luciferin-luciferase system is well known, the molecular control of bioluminescence, the biosynthetic route of luciferin, and the molecular differentiation of photogenic tissue in Elateridae remain enigmatic. With the aim to improve the knowledge about these questions, we performed the RNA-Seq analysis of lanterns and fat body of the Brazilian click beetle larvae: Pyrearinus fragilis (luminescent) and Conoderus sp. (non-luminescent) and compared the gene products contents and their abundance among these tissues. For the first time, we showed that the fat body of luminescent click beetle displays luminescence. We also analyzed some gene products expression patterns in lantern and fat body of Elateridae. Several of them, which are potentially involved with bioluminescence and its control are also found in the transcriptional profile of the firefly Aspisoma lineatum (Lampyridae) and the railroad worm Phrixothrix hirtus (Phengodidae). These gene products were observed in both lanterns and fat body of elaterid species, however with low abundance in the fat body. The results indicate that in Elateridae, the bioluminescent tissue also originated from the fat body. In P. fragilis, we observed two luciferase/luciferase-like enzymes with high abundance, one in the lantern and a different one in the fat body, indicating the possibility of two distinct isoenzymes involved in the bioluminescence in the lantern and fat body of Elateridae species, such as observed in fireflies.
机译：Superfamily Elateroidea中的生物发光由分布在身体不同部位的特定光器官，这取决于寿命和家庭。然而，荧光素 - 荧光素酶系统是众所周知的，而且生物发光的分子控制，荧光素的生物合成途径以及Elateridae中的镜子的分子分化仍然是神秘的。旨在提高关于这些问题的知识，我们进行了巴西兰西尼亚灯笼和脂肪体的RNA-SEQ分析点击甲虫幼虫：Pyrearinus fragilis（发光）和Conoderus sp。 （非发光）并比较这些组织中的基因产物含量及其丰富。我们首次表明发光的脂肪身体点击甲虫显示发光。我们还分析了Elateridae灯和脂肪体中的一些基因产品表达模式。其中几个可能涉及生物发光及其对照的萤火虫aspisoomaatum（兰迪里夫）和铁路蠕虫皮西克斯Hirtus（Phengodidae）的转录剖面中也发现。这些基因产物在灯笼和脂肪体的灯体和脂肪体中观察到，然而在脂肪体内具有低丰度。结果表明，在Elateridae中，生物发光组织也起源于脂肪体。在P. Fragilis中，我们观察到具有高丰度的荧光素酶/荧光素样酶样酶，其中一个在脂肪体中，不同的一种在脂肪体中，表明两个不同同工酶的可能性涉及榆树灯和脂肪体中的生物发光和脂肪体中物种，如在萤火虫中观察。

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