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首页> 外文期刊>Acta Horticulturae >Development of a serological assay for detecting serologically diverse banana streak virus isolates.
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Development of a serological assay for detecting serologically diverse banana streak virus isolates.

机译：用于检测血清学上不同的香蕉条纹病毒分离株的血清学检测方法的开发。

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Banana streak virus (BSV), a double-stranded DNA plant virus that belongs to the genus Badnavirus, causes banana streak disease. This disease occurs wherever bananas and plantains are grown. Symptoms include yellow and necrotic leaf streaks, lethalstem necrosis, pseudostem splitting and 'choking'. BSV is primarily disseminated in vegetatively propagated planting material such as corms, and tissue culture-derived plants. BSV is also seed transmitted, an important consideration in breeding programmes. Successful management of BSV requires accurate detection of the virus to avoid the use of infected material for planting, tissue culture propagation, and breeding. Therefore, it is important to develop a reliable method for detecting the virus. However, BSV has high genomic and serologic variability which makes detection difficult. To obtain BSV specific broad spectrum polyclonal antibodies, rabbit and chicken polyclonal antibodies were prepared against sugarcane bacilliform virus (ScBV), a virus with close serological relationships to BSV. ScBV was isolated from a mixture of 32 sugarcane cultivars representing the widest serological diversity of this virus. In addition, polyclonal antibodies were generated to a BSV isolate from the banana cultivar'Mysore', because this isolate is not serologically related to ScBV. Using the resulting polyclonal antibodies three of five serologically unrelated BSV isolates were detected using the double antibody sandwich enzyme immunoassay (DAS-EIA). However, immunosorbent electron microscopy showed that all five tissue samples tested in the DAS-EIA had virus particles. The five BSV isolates were detected using the triple antibody sandwich enzyme immunoassay (TAS-EIA). These results indicate that TAS-EIA is moresensitive and more likely than DAS-EIA to detect a wider range of BSV isolates in infected banana and plantain material using the polyclonal antibodies produced.
机译：香蕉条纹病毒（BSV）是一种双链DNA植物病毒，属于Badnavirus属，它引起香蕉条纹病。这种疾病发生在种植香蕉和大蕉的任何地方。症状包括黄色和坏死的叶纹，致死性坏死，假性干裂和“窒息”。 BSV主要散布在无性繁殖的种植材料中，例如球茎和组织培养衍生的植物。 BSV也通过种子传播，这是育种计划中的重要考虑因素。 BSV的成功管理需要准确检测病毒，以避免将受感染的材料用于种植，组织培养繁殖和繁殖。因此，开发检测病毒的可靠方法很重要。然而，BSV具有高的基因组和血清学变异性，这使得检测困难。为了获得BSV特异的广谱多克隆抗体，制备了针对甘蔗杆菌型病毒（ScBV）的兔和鸡多克隆抗体，该病毒与BSV具有密切的血清学关系。从代表该病毒最广泛的血清学多样性的32个甘蔗品种的混合物中分离了ScBV。另外，针对香蕉栽培种“迈索尔”的BSV分离株产生了多克隆抗体，因为该分离株在血清学上与ScBV无关。使用所得的多克隆抗体，使用双抗体夹心酶免疫分析（DAS-EIA）检测了五个血清学无关的BSV分离物中的三个。但是，免疫吸附电子显微镜显示，在DAS-EIA中测试的所有五个组织样本均具有病毒颗粒。使用三抗体夹心酶免疫分析法（TAS-EIA）检测了五个BSV分离物。这些结果表明，使用产生的多克隆抗体，TAS-EIA比DAS-EIA更敏感，并且更有可能在感染的香蕉和车前草材料中检测到更广泛的BSV分离株。

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