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首页> 外文期刊>Acta Horticulturae >Results of ELISA tests of plum trees artificially infected with plum pox virus (PPV)
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Results of ELISA tests of plum trees artificially infected with plum pox virus (PPV)

机译：人工感染李痘病毒（PPV）的李树的ELISA试验结果

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An orchard of plum germplasm with 207 cultivars and clones was planted during the autumn of 1990 in an isolated area in the Czech Republic using 1-year-old trees on Myrobalan rootstocks. From every accession, 2 trees were planted. All trees were indexed by ELISA for plum pox potyvirus (PPV) infection during the beginning of the first growing season (1991). In Aug. 1991, all trees were inoculated with a virulent isolate of PPV by grafting 3 buds from an infected Prunus domestica tree on every tree stem. The inoculum source was free from prune dwarf ilarvirus and prunus necrotic ringspot ilarvirus. In the year after inoculation (1992), the trees without PPV symptoms and those without typical PPV symptoms were indexed by ELISA. From the total numberof 196 trees tested, 117 were infected with PPV and 79 were disease free. In the following year (1993), all 446 experimental trees including those with PPV symptoms on leaves were indexed by ELISA. A total of 344 trees gave positive reactions and the remaining 102 were healthy. The trees infected with PPV were classified into 5 groups according to their values of extinction, which were calculated by subtracting a border value of extinction of negative samples from the actually measured extinction of every tested tree. In 1992, 21.9% of tested trees were rated into the group 1 with the lowest extinction ranging between 0.01 and 0.05 (extinction value above border value of extinction from negative controls), but in 1993, only 5.8% of tested trees could be classified into this group. In 1992, 16.3% of trees were rated into the group 5 with the highest values of extinction (1.01-1.5), and in 1993, as much as 36.1% of tested trees fell in this category. In both years, the tests were performed using leavescollected in Sep. or Oct. when the majority of trees manifested distinct PPV on leaves.
机译：1990年秋季，使用Myrobalan砧木上的1岁树种，在捷克共和国的一个偏远地区种植了具有207个栽培品种和无性系的李子种质园。每次添加都种了2棵树。在第一个生长季节开始（1991年）期间，通过ELISA对所有树木进行了李子痘病毒（PPV）感染的索引。 1991年8月，在每棵树的茎上嫁接了3株来自受感染的李属李树的芽，从而在所有树上接种了PPV的强毒分离株。接种源不含梅花矮小艾拉病毒和李坏死环斑艾拉病毒。接种后的第二年（1992年），通过ELISA对没有PPV症状和没有典型PPV症状的树木进行索引。在测试的196棵树木总数中，有117棵被PPV感染，有79棵无病。在第二年（1993年），所有446棵实验树，包括叶片上有PPV症状的树都通过ELISA进行索引。共有344棵树产生了积极的反应，其余102棵是健康的。根据PPV的消光值，将被PPV感染的树分为5组，其计算方法是从每棵测试树的实际测得的消光值中减去阴性样品的消光值。在1992年，21.9％的受测树木被归为第一类，其最低灭绝范围为0.01到0.05（消光值高于阴性对照的灭绝边界值），但在1993年，只有5.8％的被测树木被分类为这个小组。在1992年，16.3％的树木被归为最高灭绝值（1.01-1.5）的第5组，而在1993年，多达36.1％的被测试树木属于此类。在这两年中，当大多数树木在叶子上表现出明显的PPV时，都是使用9月或10月收集的叶子进行测试。

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