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首页> 外文期刊>Acta Horticulturae >Cucurbit-potyvirus interactions influencing virus movement and accumulation.
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Cucurbit-potyvirus interactions influencing virus movement and accumulation.

机译：葫芦-马铃薯病毒相互作用影响病毒的移动和积累。

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Cucurbit crops are subject to severe losses due to several potyviruses including the highly aggressive zucchini yellow mosaic virus (ZYMV). We sought to examine potyvirus/host interactions resulting in successful resistance or infection, and to testthe role of the variable amino terminus (NT) of the coat protein (CP) in the infection process. Analysis of patterns of virus accumulation in the ZYMV-resistant cucumber (Cucumis sativus) genotype Dina-1 indicated that expression of resistance is developmentally controlled and is due to a block in virus movement. After initial replication and movement out of the cotyledon, virus remains localized to the immediate area surrounding class III veins. Switches of CP domains, with an infectious clone (ZYMV-NAA) that does not cause veinal chlorosis, indicated that the resistance response likely involves interaction with the CP-NT. Switches of the CP-NT with other potyviruses indicated, that despite extensive variability, the heterologous CP-NTs could facilitate systemic infection in ZYMV hosts, but did not extend host range to non-hosts. Following initial infection, susceptible squash (Cucurbita pepo) or cucumber plants infected with tobacco etch virus-ZYMV hybrids exhibited a distinct recovery phenotype ofreduced symptoms, virus titre, and virus-specific protection against secondary infection, suggesting that recognition of the non-adapted CP-NT leads to initiation of host defense responses.
机译：葫芦类作物因几种马铃薯病毒而遭受严重损失，其中包括高度侵略性的西葫芦黄色花叶病毒（ZYMV）。我们试图检查导致成功的抗药性或感染的马铃薯病毒/宿主相互作用，并测试外壳蛋白（CP）的可变氨基末端（NT）在感染过程中的作用。对ZYMV抗性黄瓜（Cucumis sativus）基因型Dina-1的病毒积累模式的分析表明，抗性的表达受到发育控制，并且是由于病毒运动受阻。最初复制并移出子叶后，病毒仍定位在III类静脉周围的直接区域。具有感染性克隆（ZYMV-NAA）且不会引起静脉萎黄的CP结构域的切换表明，耐药性应答可能涉及与CP-NT的相互作用。 CP-NT与其他马铃薯病毒的切换表明，尽管变异很大，但异源CP-NT可以促进ZYMV宿主的系统感染，但不会将宿主范围扩展至非宿主。初次感染后，感染烟草蚀刻病毒-ZYMV杂种的易感染南瓜（黄瓜）或黄瓜植株表现出明显的恢复表型，具有减轻的症状，病毒滴度和针对继发感染的病毒特异性保护作用，表明对非适应性CP的识别-NT导致发起宿主防御反应。

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