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首页> 外文期刊>Biomacromolecules >Distribution of Ionizable Groups in Polyampholyte Microgels Controls Interactions with Captured Proteins: From Blockade and 'Levitation' to Accelerated Release
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Distribution of Ionizable Groups in Polyampholyte Microgels Controls Interactions with Captured Proteins: From Blockade and 'Levitation' to Accelerated Release

机译：聚酰晶微凝胶中可电离基团的分布对照与捕获蛋白质的相互作用：从阻滞和“悬浮”加速释放

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摘要

A striking discovery in our work is that the distribution of ionizable groups in polyampholyte microgels (random and core-shell) controls the interactions with the captured proteins. Polyampholyte microgels are capable to switch reversibly their charges from positive to negative depending on pH. In this work, we synthesized differently structured polyampholyte microgels with controlled amounts and different distribution of acidic and basic moieties as colloidal carriers to study the loading and release of the model protein cytochrome c (cyt-c). Polyampholyte microgels were first loaded with cyt-c using the electrostatic attraction under pH 8 when the microgels were oppositely charged with respect to the protein. Then the protein release was investigated under different pH (3, 6, and 8) both with experimental methods and molecular dynamics simulations. For microgels with a random distribution of ionizable groups complete and accelerated (compared to polyelectrolyte counterpart) release of cyt-c was observed due to electrostatic repulsive interactions. For core- shell structured microgels with defined ionizable groups, it was possible to entrap the protein inside the neutral core through the formation of a positively charged shell, which acts as an electrostatic potential barrier. We postulate that this discovery allows the design of functional colloidal carriers with programmed release kinetics for applications in drug delivery, catalysis, and biomaterials.
机译：在我们的作品中醒目的发现是聚酰晶微粒中可电离电离基团（随机和核心壳）的分布控制与捕获的蛋白质的相互作用。聚酰胺微凝胶能够根据pH值可逆地切换它们的电荷。在这项工作中，我们用控制量和酸性和基本部分的不同分布合成了不同结构化的聚酰晶微凝胶作为胶体载体，以研究模型蛋白细胞色素C（Cyt-C）的装载和释放。当微凝胶相对于蛋白质相反地，首先使用pH8下的静电吸引，首先使用pH8的静电吸引来装载多聚晶晶硅。然后通过实验方法和分子动力学模拟在不同pH（3,6和8）下进行蛋白质释放。对于具有可电离基团随机分布的微凝胶，由于静电排斥相互作用，观察到Cyt-C的释放（与聚电解质对应物相比）释放。对于具有限定可电离基团的核心壳结构微凝胶，可以通过形成带正电荷的壳体捕获中性芯内的蛋白质，其充当静电潜在屏障。我们假设该发现允许设计具有编程释放动力学的功能性胶体载体，用于药物递送，催化和生物材料中的应用。

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  • 来源
    《Biomacromolecules》 |2019年第4期|共14页
  • 作者

    Xu Wenjing; Rudov Andrey A.; Schroeder Ricarda; Portnov Ivan V.; Richtering Walter; Potemkin Igor I.; Pich Andrij;

  • 作者单位

    DWI Leibniz Inst Interact Mat eV Forckenbeckstr 50 D-52074 Aachen Germany;

    DWI Leibniz Inst Interact Mat eV Forckenbeckstr 50 D-52074 Aachen Germany;

    DWI Leibniz Inst Interact Mat eV Forckenbeckstr 50 D-52074 Aachen Germany;

    Lomonosov Moscow State Univ Phys Dept GSP-1 1-2 Leninskiye Gory Moscow 119991 Russia;

    Rhein Westfal TH Aachen Inst Phys Chem Landoltweg 2 D-52056 Aachen Germany;

    DWI Leibniz Inst Interact Mat eV Forckenbeckstr 50 D-52074 Aachen Germany;

    DWI Leibniz Inst Interact Mat eV Forckenbeckstr 50 D-52074 Aachen Germany;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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