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首页> 外文期刊>Acta Horticulturae >A microcin produced by a strain of Erwinia herbicola is involved in biological control of fire blight and soft rot caused by Erwinia sp.
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A microcin produced by a strain of Erwinia herbicola is involved in biological control of fire blight and soft rot caused by Erwinia sp.

机译：由草欧文氏杆菌菌株产生的微素参与了欧文氏菌引起的火疫病和软腐病的生物防治。

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摘要

Strain Eh252 of E. herbicola [Pantoea agglomerans] produces a compound which inhibits on plate the fire blight pathogen (E. amylovora) and strains of Erwinia species responsible for soft rot on vegetables and ornamentals in the field and during storage (E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and E. chrysanthemi). Determination of the biochemical characteristics of this compound as well as DNA sequence analysis of the genes necessary for its production indicate that thiscompound is a small peptide with a modification such as adenylation. Its production is not regulated by the SOS system and it retains activity after exposure to extreme pH and extreme temperature. All these characteristics indicate that this compound belongs to a family of antibiotics called microcin. Transposon-induced mutants of Eh252 which do not produce this microcin were not able to protect immature pear fruits against fire blight as well as the wild-type strain. Similarly, these mutants did not protect carrot or potato (cultivars Rua and Ilam Hardy) slices as well as Eh252 against soft rot. The ability to control soft rot and fire blight was restored when the mutants were complemented for microcin production. This is the first microcin produced by a strain of E. herbicola and the first time a microcin has been involved in the control of plant pathogenic bacteria. The genes for production of this microcin have been cloned, and can be expressed in other bacteria than E. herbicola. This allows us to develop a strategy of biological control for fire blight and bacterial soft rot based on superior biocontrol agents to which we would confer the ability to produce this microcin.
机译：草药大肠杆菌（E. Herbicola）[Pantoea agloglomerans]的菌株Eh252产生了一种化合物，该化合物可在板上抑制火疫病病原体（Amylovora）和在田间和贮藏期间对蔬菜和装饰物造成软腐的欧文氏菌菌株（Carotovora subsp） 。。。。。。。。。。。该化合物生化特性的测定以及其产生所必需基因的DNA序列分析表明，该化合物是带有修饰（如腺苷酸化）的小肽。它的生产不受SOS系统的调节，并且在暴露于极端pH和极端温度后仍保持活性。所有这些特征表明该化合物属于称为微素的抗生素家族。转座子诱导的Eh252突变体不产生这种微霉素，不能保护未成熟的梨果实免受火疫病和野生型菌株的侵害。同样，这些突变体也不能保护胡萝卜或马铃薯（Rua和Ilam Hardy品种）切片以及Eh252免受软腐。当突变体被补充用于微素生产时，恢复了控制软腐和火疫病的能力。这是由E. herbicola菌株产生的第一个微素，也是微素第一次参与植物病原菌的控制。已经克隆了产生这种微霉素的基因，并且可以在除草杆菌外的其他细菌中表达。这使我们能够基于优异的生物防治剂制定一项针对火疫病和细菌性软腐病的生物防治策略，并赋予其生产这种微素的能力。

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