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首页> 外文期刊>Acta Horticulturae >Viroid-like RNAs associated with some apricot fruit disorders
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Viroid-like RNAs associated with some apricot fruit disorders

机译：类病毒RNA与一些杏果实疾病有关

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In Campania, Italy, 2 apricot diseases were discovered of cultivars Bulida and Tyrinthos grafted on Myrobalan rootstocks. The Bulida disease, characterized by the presence of brown-reddish round spots and/or concentric rings, is graft-transmissibleand resembles apricot ring pox described in the USA and Canada. The diseased Tyrinthos trees produce deformed fruits showing depressed green spots and few peculiar symptoms on leaves. Transmission studies, started in 1992 by grafting diseased bark chipsonto 2-year-old Tyrinthos seedlings, are in progress. In spite of the constant detection of apple chlorotic leaf spot trichovirus (ACLSV) in diseased trees of both cultivars, further investigations were carried out on the aetiology of the disorders. In 1987, 5 Bulida seedlings infected with ACLSV, isolated on Chenopodium quinoa, by approach grafting and/or inserting C. quinoa tissues underneath the bark, produced fruits in 1992 showing reddish lines and rings. Using nucleic acid extraction and viroid RNAs detection procedure, viroid-like RNAs were detected in leaves of diseased Bulida and Tyrinthos. Electrophoretic mobility of the RNAs under denaturing conditions was similar to that of hop stunt viroid (HSVd) recently detected in apricot. It is suggested that the described disorders could be caused by a simultaneous infection of ACLSV and a viroid.
机译：在意大利的坎帕尼亚（Campania），发现了嫁接在Myrobalan砧木上的Bulida和Tyrinthos品种的2种杏病。以存在棕红色的圆形斑点和/或同心环为特征的布利达病是可移植的，类似于美国和加拿大描述的杏环痘。患病的蒂林索斯（Tyrinthos）树产生畸形的果实，显示出凹陷的绿色斑点，并且在叶子上几乎没有奇特的症状。传播研究始于1992年，开始时将病态的树皮屑嫁接到2岁的Tyrinthos幼苗上。尽管在两个品种的患病树中都不断检测到苹果绿化叶斑癣病毒（ACLSV），但仍对该病的病因进行了进一步研究。 1987年，通过在藜皮上嫁接和/或插入藜麦组织分离了5株被ACLSV感染的Bulida幼苗，方法是在树皮下嫁接和/或插入藜麦。在1992年，结果显示红线和环。使用核酸提取和类病毒RNA检测程序，在患病的布利达和蒂林斯州的叶片中检测到类病毒RNA。在变性条件下，RNA的电泳迁移率与最近在杏子中检测到的跳特技类病毒（HSVd）相似。建议所描述的疾病可能是由同时感染ACLSV和类病毒引起的。

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